{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["40(1)"],"submitter":["Amann LF"],"funding":["Universität Hamburg"],"pubmed_abstract":["<h4>Purpose</h4>This study aimed to examine the degradation of tigecycline in Mueller Hinton broth (ca-MHB), as knowledge about bacterial susceptibility is key for therapeutic decisions.<h4>Methods</h4>Antioxidative stabilizers were evaluated on tigecycline stability in a quantitative chromatography assay and tigecycline induced kill against Staphylococcus aureus (ATCC29213) was determined in time kill studies.<h4>Results</h4>Ascorbic acid caused rapid degradation of tigecycline and resulted in loss of antibacterial activity. Tigecycline was stabilized in aged broth by 2% pyruvate and bacterial growth, and tigecycline killing was similar to fresh broth without supplementation, but independent of age.<h4>Conclusion</h4>Our results underline the importance of using freshly prepared ca-MHB or"],"journal":["European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology"],"pagination":["215-218"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7782409"],"repository":["biostudies-literature"],"pubmed_title":["Stability studies with tigecycline in bacterial growth medium and impact of stabilizing agents."],"pmcid":["PMC7782409"],"pubmed_authors":["Rathke M","Wicha SG","Broeker A","Riedner M","Vicente ER","Amann LF"],"additional_accession":[]},"is_claimable":false,"name":"Stability studies with tigecycline in bacterial growth medium and impact of stabilizing agents.","description":"<h4>Purpose</h4>This study aimed to examine the degradation of tigecycline in Mueller Hinton broth (ca-MHB), as knowledge about bacterial susceptibility is key for therapeutic decisions.<h4>Methods</h4>Antioxidative stabilizers were evaluated on tigecycline stability in a quantitative chromatography assay and tigecycline induced kill against Staphylococcus aureus (ATCC29213) was determined in time kill studies.<h4>Results</h4>Ascorbic acid caused rapid degradation of tigecycline and resulted in loss of antibacterial activity. Tigecycline was stabilized in aged broth by 2% pyruvate and bacterial growth, and tigecycline killing was similar to fresh broth without supplementation, but independent of age.<h4>Conclusion</h4>Our results underline the importance of using freshly prepared ca-MHB or","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Jan","modification":"2025-05-31T22:41:20.38Z","creation":"2025-05-31T22:41:20.38Z"},"accession":"S-EPMC7782409","cross_references":{"pubmed":["32720091"],"doi":["10.1007/s10096-020-03970-0"]}}