{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["8"],"submitter":["Anbazhagan R"],"funding":["Eunice Kennedy Shriver National Institute of Child Health and Human Development"],"pubmed_abstract":["GRTH/DDX25 is a member of the DEAD-box family of RNA helicases that play an essential role in spermatogenesis. GRTH knock-in (KI) mice with the human mutant GRTH gene (R242H) show loss of the phospho-species from cytoplasm with preservation of the non-phospho form in the cytoplasm and nucleus. GRTH KI mice are sterile and lack elongated spermatids and spermatozoa, with spermatogenic arrest at step 8 of round spermatids which contain chromatoid body (CB) markedly reduced in size. We observed an absence of phospho-GRTH in CB of GRTH KI mice. RNA-Seq analysis of mRNA isolated from CB revealed that 1,421 genes show differential abundance, of which 947 genes showed a decrease in abundance and 474 genes showed an increase in abundance in GRTH KI mice. The transcripts related to spermatid develop"],"journal":["Frontiers in cell and developmental biology"],"pagination":["580019"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7786181"],"repository":["biostudies-literature"],"pubmed_title":["Role of Phosphorylated Gonadotropin-Regulated Testicular RNA Helicase (GRTH/DDX25) in the Regulation of Germ Cell Specific mRNAs in Chromatoid Bodies During Spermatogenesis."],"pmcid":["PMC7786181"],"pubmed_authors":["Kavarthapu R","Coon SL","Dufau ML","Anbazhagan R"],"additional_accession":[]},"is_claimable":false,"name":"Role of Phosphorylated Gonadotropin-Regulated Testicular RNA Helicase (GRTH/DDX25) in the Regulation of Germ Cell Specific mRNAs in Chromatoid Bodies During Spermatogenesis.","description":"GRTH/DDX25 is a member of the DEAD-box family of RNA helicases that play an essential role in spermatogenesis. GRTH knock-in (KI) mice with the human mutant GRTH gene (R242H) show loss of the phospho-species from cytoplasm with preservation of the non-phospho form in the cytoplasm and nucleus. GRTH KI mice are sterile and lack elongated spermatids and spermatozoa, with spermatogenic arrest at step 8 of round spermatids which contain chromatoid body (CB) markedly reduced in size. We observed an absence of phospho-GRTH in CB of GRTH KI mice. RNA-Seq analysis of mRNA isolated from CB revealed that 1,421 genes show differential abundance, of which 947 genes showed a decrease in abundance and 474 genes showed an increase in abundance in GRTH KI mice. The transcripts related to spermatid develop","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020","modification":"2026-04-07T20:34:33.596Z","creation":"2025-04-04T07:16:28.688Z"},"accession":"S-EPMC7786181","cross_references":{"pubmed":["33425888"],"doi":["10.3389/fcell.2020.580019"]}}