{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["25"],"submitter":["Kubouchi K"],"funding":["Ministry of Education, Culture, Sports, Science and Technology"],"pubmed_abstract":["The role of Protein Kinase N2 (PKN2, also known as PRK2/PKNγ) in cell aggregate/spheroid formation in suspension culture was investigated using immortalized fibroblasts established from <i>PKN2</i> <sup>flox/flox</sup> mouse embryos. <i>PKN2</i> <sup>flox/flox</sup> cells formed cell aggregates in flat bottom low attachment well plates, such as 2% agar and poly-2-hydroxyethymethacrylate coated plates, however, Cre;<i>PKN2</i> <sup>flox/flox</sup> cells in which PKN2 was depleted by the introduction of Cre-recombinase rarely formed aggregates. Time-lapse analysis revealed that the velocity of Cre;<i>PKN2</i> <sup>flox/flox</sup> cell motility was significantly lower than that of <i>PKN2</i> <sup>flox/flox</sup> in a low attachment flat-bottom plate, which likely resulted in a lower cell-cel"],"journal":["Biochemistry and biophysics reports"],"pagination":["100895"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7787963"],"repository":["biostudies-literature"],"pubmed_title":["PKN2 is involved in aggregation and spheroid formation of fibroblasts in suspension culture by regulating cell motility and <i>N-cadherin</i> expression."],"pmcid":["PMC7787963"],"pubmed_authors":["Mukai H","Kubouchi K"],"additional_accession":[]},"is_claimable":false,"name":"PKN2 is involved in aggregation and spheroid formation of fibroblasts in suspension culture by regulating cell motility and <i>N-cadherin</i> expression.","description":"The role of Protein Kinase N2 (PKN2, also known as PRK2/PKNγ) in cell aggregate/spheroid formation in suspension culture was investigated using immortalized fibroblasts established from <i>PKN2</i> <sup>flox/flox</sup> mouse embryos. <i>PKN2</i> <sup>flox/flox</sup> cells formed cell aggregates in flat bottom low attachment well plates, such as 2% agar and poly-2-hydroxyethymethacrylate coated plates, however, Cre;<i>PKN2</i> <sup>flox/flox</sup> cells in which PKN2 was depleted by the introduction of Cre-recombinase rarely formed aggregates. Time-lapse analysis revealed that the velocity of Cre;<i>PKN2</i> <sup>flox/flox</sup> cell motility was significantly lower than that of <i>PKN2</i> <sup>flox/flox</sup> in a low attachment flat-bottom plate, which likely resulted in a lower cell-cel","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Mar","modification":"2025-06-01T00:57:27.37Z","creation":"2025-06-01T00:57:27.37Z"},"accession":"S-EPMC7787963","cross_references":{"pubmed":["33437883"],"doi":["10.1016/j.bbrep.2020.100895"]}}