biostudies-literatureUnknown25Kubouchi KMinistry of Education, Culture, Sports, Science and TechnologyThe role of Protein Kinase N2 (PKN2, also known as PRK2/PKNγ) in cell aggregate/spheroid formation in suspension culture was investigated using immortalized fibroblasts established from <i>PKN2</i> ^flox/flox mouse embryos. <i>PKN2</i> ^flox/flox cells formed cell aggregates in flat bottom low attachment well plates, such as 2% agar and poly-2-hydroxyethymethacrylate coated plates, however, Cre;<i>PKN2</i> ^flox/flox cells in which PKN2 was depleted by the introduction of Cre-recombinase rarely formed aggregates. Time-lapse analysis revealed that the velocity of Cre;<i>PKN2</i> ^flox/flox cell motility was significantly lower than that of <i>PKN2</i> ^flox/flox in a low attachment flat-bottom plate, which likely resulted in a lower cell-celBiochemistry and biophysics reports100895https://www.ebi.ac.uk/biostudies/studies/S-EPMC7787963biostudies-literaturePKN2 is involved in aggregation and spheroid formation of fibroblasts in suspension culture by regulating cell motility and <i>N-cadherin</i> expression.PMC7787963Mukai HKubouchi KfalsePKN2 is involved in aggregation and spheroid formation of fibroblasts in suspension culture by regulating cell motility and <i>N-cadherin</i> expression.The role of Protein Kinase N2 (PKN2, also known as PRK2/PKNγ) in cell aggregate/spheroid formation in suspension culture was investigated using immortalized fibroblasts established from <i>PKN2</i> ^flox/flox mouse embryos. <i>PKN2</i> ^flox/flox cells formed cell aggregates in flat bottom low attachment well plates, such as 2% agar and poly-2-hydroxyethymethacrylate coated plates, however, Cre;<i>PKN2</i> ^flox/flox cells in which PKN2 was depleted by the introduction of Cre-recombinase rarely formed aggregates. Time-lapse analysis revealed that the velocity of Cre;<i>PKN2</i> ^flox/flox cell motility was significantly lower than that of <i>PKN2</i> ^flox/flox in a low attachment flat-bottom plate, which likely resulted in a lower cell-cel2021-01-01T00:00:00Z2021 Mar2025-06-01T00:57:27.37Z2025-06-01T00:57:27.37ZS-EPMC77879633343788310.1016/j.bbrep.2020.100895