biostudies-literatureOlkowicz MFundacja na rzecz Nauki PolskiejNarodowe Centrum Nauki6https://www.ebi.ac.uk/biostudies/studies/S-EPMC7789501biostudies-literatureUnknown19(1)<h4>Background</h4>Dyslipidaemia is a major risk factor for atherosclerosis and cardiovascular diseases. The molecular mechanisms that translate dyslipidaemia into atherogenesis and reliable markers of its progression are yet to be fully elucidated. To address this issue, we conducted a comprehensive metabolomic and proteomic analysis in an experimental model of dyslipidaemia and in patients with familial hypercholesterolemia (FH).<h4>Methods</h4>Liquid chromatography/mass spectrometry (LC/MS) and immunoassays were used to find out blood alterations at metabolite and protein levels in dyslipidaemic ApoE^-/-/LDLR^-/- mice and in FH patients to evaluate their human relevance.<h4>Results</h4>We identified 15 metabolites (inhibitors and substrates of nitric oxide synthase (Journal of translational medicineMulti-omic signatures of atherogenic dyslipidaemia: pre-clinical target identification and validation in humans.PMC7789501POIR.04.04.00‐00‐5CAC/17–002016/23/B/NZ4/038772018/29/B/NZ7/01684Kochan ZSzupryczynska NSmolenski RTDadlez MOlkowicz MCzyzynska-Cichon IDebski JChlopicki SKostogrys RBfalseMulti-omic signatures of atherogenic dyslipidaemia: pre-clinical target identification and validation in humans.<h4>Background</h4>Dyslipidaemia is a major risk factor for atherosclerosis and cardiovascular diseases. The molecular mechanisms that translate dyslipidaemia into atherogenesis and reliable markers of its progression are yet to be fully elucidated. To address this issue, we conducted a comprehensive metabolomic and proteomic analysis in an experimental model of dyslipidaemia and in patients with familial hypercholesterolemia (FH).<h4>Methods</h4>Liquid chromatography/mass spectrometry (LC/MS) and immunoassays were used to find out blood alterations at metabolite and protein levels in dyslipidaemic ApoE^-/-/LDLR^-/- mice and in FH patients to evaluate their human relevance.<h4>Results</h4>We identified 15 metabolites (inhibitors and substrates of nitric oxide synthase (2021-01-01T00:00:00Z2021 Jan2026-05-03T04:56:47.031Z2025-02-18T23:13:11.14ZS-EPMC77895013340755510.1186/s12967-020-02663-8