{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zhou P"],"funding":["Gansu Province Science Foundation for Youths","Fundamental Research Funds for the Central Universities","Young Elite Scientist Sponsorship Program by CSA","Lanzhou University Hospital of Stomatology Research Support Fund","Chengguan District Science and Technology Project","the National Natural Science Foundation of China"],"pagination":["41"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7792045"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12(1)"],"pubmed_abstract":["Background
Derivation of osteoblast-like cells from human pluripotent stem cells (hPSCs) is a popular topic in bone tissue engineering. Although many improvements have been achieved, the low induction efficiency because of spontaneous differentiation hampers their applications. To solve this problem, a detailed understanding of the osteogenic differentiation process of hPSCs is urgently needed.Methods
Monolayer cultured human embryonic stem cells and human-induced pluripotent stem cells were differentiated in commonly applied serum-containing osteogenic medium for 35 days. In addition to traditional assays such as cell viability detection, reverse transcription-polymerase chain reaction, immunofluorescence, and alizarin red staining, we also applied studies of cell counting"],"journal":["Stem cell research & therapy"],"pubmed_title":["Establishing a deeper understanding of the osteogenic differentiation of monolayer cultured human pluripotent stem cells using novel and detailed analyses."],"pmcid":["PMC7792045"],"funding_grant_id":["lzujbky-2018-27","lzujbky-2015-295","2018-7-6","LZUKQKY-2019-Y10","No. 81571824","lzukqky-2019-t9","No.2018QNRC001","18JR3RA295"],"pubmed_authors":["Shi JM","Song YM","Lan F","Zhou P","Feng F","Song JE","Han Y","Li HJ","Zhang R","Wang JL"],"additional_accession":[]},"is_claimable":false,"name":"Establishing a deeper understanding of the osteogenic differentiation of monolayer cultured human pluripotent stem cells using novel and detailed analyses.","description":"Background
Derivation of osteoblast-like cells from human pluripotent stem cells (hPSCs) is a popular topic in bone tissue engineering. Although many improvements have been achieved, the low induction efficiency because of spontaneous differentiation hampers their applications. To solve this problem, a detailed understanding of the osteogenic differentiation process of hPSCs is urgently needed.Methods
Monolayer cultured human embryonic stem cells and human-induced pluripotent stem cells were differentiated in commonly applied serum-containing osteogenic medium for 35 days. In addition to traditional assays such as cell viability detection, reverse transcription-polymerase chain reaction, immunofluorescence, and alizarin red staining, we also applied studies of cell counting","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Jan","modification":"2025-04-18T17:04:33.572Z","creation":"2021-02-20T20:50:28Z"},"accession":"S-EPMC7792045","cross_references":{"pubmed":["33413612"],"doi":["10.1186/s13287-020-02085-9"]}}