biostudies-literatureZhou PGansu Province Science Foundation for YouthsFundamental Research Funds for the Central UniversitiesYoung Elite Scientist Sponsorship Program by CSALanzhou University Hospital of Stomatology Research Support FundChengguan District Science and Technology Projectthe National Natural Science Foundation of China41https://www.ebi.ac.uk/biostudies/studies/S-EPMC7792045biostudies-literatureUnknown12(1)<h4>Background</h4>Derivation of osteoblast-like cells from human pluripotent stem cells (hPSCs) is a popular topic in bone tissue engineering. Although many improvements have been achieved, the low induction efficiency because of spontaneous differentiation hampers their applications. To solve this problem, a detailed understanding of the osteogenic differentiation process of hPSCs is urgently needed.<h4>Methods</h4>Monolayer cultured human embryonic stem cells and human-induced pluripotent stem cells were differentiated in commonly applied serum-containing osteogenic medium for 35 days. In addition to traditional assays such as cell viability detection, reverse transcription-polymerase chain reaction, immunofluorescence, and alizarin red staining, we also applied studies of cell countingStem cell research & therapyEstablishing a deeper understanding of the osteogenic differentiation of monolayer cultured human pluripotent stem cells using novel and detailed analyses.PMC7792045lzujbky-2018-27lzujbky-2015-2952018-7-6LZUKQKY-2019-Y10No. 81571824lzukqky-2019-t9No.2018QNRC00118JR3RA295Shi JMSong YMLan FZhou PFeng FSong JEHan YLi HJZhang RWang JLfalseEstablishing a deeper understanding of the osteogenic differentiation of monolayer cultured human pluripotent stem cells using novel and detailed analyses.<h4>Background</h4>Derivation of osteoblast-like cells from human pluripotent stem cells (hPSCs) is a popular topic in bone tissue engineering. Although many improvements have been achieved, the low induction efficiency because of spontaneous differentiation hampers their applications. To solve this problem, a detailed understanding of the osteogenic differentiation process of hPSCs is urgently needed.<h4>Methods</h4>Monolayer cultured human embryonic stem cells and human-induced pluripotent stem cells were differentiated in commonly applied serum-containing osteogenic medium for 35 days. In addition to traditional assays such as cell viability detection, reverse transcription-polymerase chain reaction, immunofluorescence, and alizarin red staining, we also applied studies of cell counting2021-01-01T00:00:00Z2021 Jan2025-04-18T17:04:33.572Z2021-02-20T20:50:28ZS-EPMC77920453341361210.1186/s13287-020-02085-9