biostudies-literatureUnknown14(1)Ding YThe immobilization technology provides a potential pathway for enzyme recycling. Here, we evaluated the potential of using dextranase immobilized onto hydroxyapatite nanoparticles as a promising inorganic material. The optimal immobilization temperature, reaction time, and pH were determined to be 25 °C, 120 min, and pH 5, respectively. Dextranase could be loaded at 359.7 U/g. The immobilized dextranase was characterized by field emission gun-scanning electron microscope (FEG-SEM), X-ray diffraction (XRD), and Fourier-transformed infrared spectroscopy (FT-IR). The hydrolysis capacity of the immobilized enzyme was maintained at 71% at the 30th time of use. According to the constant temperature acceleration experiment, it was estimated that the immobilized dextranase could be stored for 99 dMaterials (Basel, Switzerland)E130https://www.ebi.ac.uk/biostudies/studies/S-EPMC7796272biostudies-literatureImmobilization of Dextranase on Nano-Hydroxyapatite as a Recyclable Catalyst.PMC7796272Dong DLyu MDing YWang CWang SZhang HZu HWang XfalseImmobilization of Dextranase on Nano-Hydroxyapatite as a Recyclable Catalyst.The immobilization technology provides a potential pathway for enzyme recycling. Here, we evaluated the potential of using dextranase immobilized onto hydroxyapatite nanoparticles as a promising inorganic material. The optimal immobilization temperature, reaction time, and pH were determined to be 25 °C, 120 min, and pH 5, respectively. Dextranase could be loaded at 359.7 U/g. The immobilized dextranase was characterized by field emission gun-scanning electron microscope (FEG-SEM), X-ray diffraction (XRD), and Fourier-transformed infrared spectroscopy (FT-IR). The hydrolysis capacity of the immobilized enzyme was maintained at 71% at the 30th time of use. According to the constant temperature acceleration experiment, it was estimated that the immobilized dextranase could be stored for 99 d2020-01-01T00:00:00Z2020 Dec2025-04-25T20:47:13.228Z2025-04-06T08:28:07.09ZS-EPMC77962723339681010.3390/ma14010130