{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Bai R"],"funding":["Natural Science Foundation of Beijing Municipality","National Natural Science Foundation of China","Non-profit Central Research Institute Fund of Chinese Academy of Medical Sciences"],"pagination":["48"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7796386"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12(1)"],"pubmed_abstract":["<h4>Introduction</h4>Spinal cord injury (SCI) is a neurological, medically incurable disorder. Human pluripotent stem cells (hPSCs) have the potential to generate neural stem/progenitor cells (NS/PCs), which hold promise in the treatment of SCI by transplantation. In our study, we aimed to establish a chemically defined culture system using serum-free medium and ascorbic acid (AA) to generate and expand long-term self-renewing neuroepithelial-like stem cells (lt-NES cells) differentiated from hPSCs effectively and stably.<h4>Methods</h4>We induced human embryonic stem cells (hESCs)/induced PSCs (iPSCs) to neurospheres using a newly established in vitro induction system. Moreover, lt-NES cells were derived from hESC/iPSC-neurospheres using two induction systems, i.e., conventional N2 medium"],"journal":["Stem cell research & therapy"],"pubmed_title":["Ascorbic acid can promote the generation and expansion of neuroepithelial-like stem cells derived from hiPS/ES cells under chemically defined conditions through promoting collagen synthesis."],"pmcid":["PMC7796386"],"funding_grant_id":["81970205","2019PT320026","Z190013"],"pubmed_authors":["Dong T","Tian L","You Y","Ma S","Lan F","Guo T","Jiang HF","Zhang S","Chang Y","Wu F","Lu WJ","Jiang Y","Li Y","Bai R","Saleem A"],"additional_accession":[]},"is_claimable":false,"name":"Ascorbic acid can promote the generation and expansion of neuroepithelial-like stem cells derived from hiPS/ES cells under chemically defined conditions through promoting collagen synthesis.","description":"<h4>Introduction</h4>Spinal cord injury (SCI) is a neurological, medically incurable disorder. Human pluripotent stem cells (hPSCs) have the potential to generate neural stem/progenitor cells (NS/PCs), which hold promise in the treatment of SCI by transplantation. In our study, we aimed to establish a chemically defined culture system using serum-free medium and ascorbic acid (AA) to generate and expand long-term self-renewing neuroepithelial-like stem cells (lt-NES cells) differentiated from hPSCs effectively and stably.<h4>Methods</h4>We induced human embryonic stem cells (hESCs)/induced PSCs (iPSCs) to neurospheres using a newly established in vitro induction system. Moreover, lt-NES cells were derived from hESC/iPSC-neurospheres using two induction systems, i.e., conventional N2 medium","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Jan","modification":"2025-05-18T11:45:55.978Z","creation":"2025-05-18T11:45:55.978Z"},"accession":"S-EPMC7796386","cross_references":{"pubmed":["33422132"],"doi":["10.1186/s13287-020-02115-6"]}}