biostudies-literatureUnknown24(1)Agrawal IMinistry of Electronics and Information technologyDepartment of Biotechnology, Ministry of Science and Technology, IndiaDopamine (DA) plays many roles in the brain, especially in movement, motivation, and reinforcement of behavior; however, its role in regulating innate immunity is not clear. Here, we show that DA can induce DNA-based extracellular traps in primary, adult, human microglia and BV2 microglia cell line. These DNA-based extracellular traps are formed independent of reactive oxygen species, actin polymerization, and cell death. These traps are functional and capture fluorescein (FITC)-tagged <i>Escherichia coli</i> even when reactive oxygen species production or actin polymerization is inhibited. We show that microglial extracellular traps are present in <i>Glioblastoma multiforme</i>. This is crucial because <i>Glioblastoma multiforme</i> cells are known to secrete DA. Our findings demonstrate iScience101968https://www.ebi.ac.uk/biostudies/studies/S-EPMC7797945biostudies-literatureDopamine induces functional extracellular traps in microglia.PMC7797945Ghatak SSharma NGupta TArvind SChakraborty DJha DJha SAgrawal ISaxena SEpari SChakraborty DBfalseDopamine induces functional extracellular traps in microglia.Dopamine (DA) plays many roles in the brain, especially in movement, motivation, and reinforcement of behavior; however, its role in regulating innate immunity is not clear. Here, we show that DA can induce DNA-based extracellular traps in primary, adult, human microglia and BV2 microglia cell line. These DNA-based extracellular traps are formed independent of reactive oxygen species, actin polymerization, and cell death. These traps are functional and capture fluorescein (FITC)-tagged <i>Escherichia coli</i> even when reactive oxygen species production or actin polymerization is inhibited. We show that microglial extracellular traps are present in <i>Glioblastoma multiforme</i>. This is crucial because <i>Glioblastoma multiforme</i> cells are known to secrete DA. Our findings demonstrate 2021-01-01T00:00:00Z2021 Jan2026-05-04T19:14:05.478Z2021-02-21T00:03:08ZS-EPMC77979453345861710.1016/j.isci.2020.101968