{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["10(1)"],"submitter":["Cheng L"],"pubmed_abstract":["Evidence suggests that metformin might be a potential candidate for breast cancer treatment. Yet, its relevant molecular mechanisms remain to be fully investigated. We found that metformin could suppress the N6-methyladenosine (m<sup>6</sup>A) level in breast cancer cells significantly. The latter has an essential role in breast cancer progression and is newly considered as a therapeutic target. In this study, we measured the m<sup>6</sup>A level by m<sup>6</sup>A colorimetric analysis and dot blot assay. We then performed qRT-PCR, western blot, MeRIP, dual-luciferase reporter assay, and others to explore the m<sup>6</sup>A-dependent pathway associated with metformin. In vivo effect of metformin was investigated using a mouse tumorigenicity model. In addition, breast cancer and normal tiss"],"journal":["Oncogenesis"],"pagination":["7"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7801402"],"repository":["biostudies-literature"],"pubmed_title":["Metformin exhibits antiproliferation activity in breast cancer via miR-483-3p/METTL3/m<sup>6</sup>A/p21 pathway."],"pmcid":["PMC7801402"],"pubmed_authors":["Shi L","Li Z","Zhou XJ","Wei JF","Huang YZ","Dai XY","Zhang X","Cheng L","Ding Q","Xu LY","Zhu YL"],"additional_accession":[]},"is_claimable":false,"name":"Metformin exhibits antiproliferation activity in breast cancer via miR-483-3p/METTL3/m<sup>6</sup>A/p21 pathway.","description":"Evidence suggests that metformin might be a potential candidate for breast cancer treatment. Yet, its relevant molecular mechanisms remain to be fully investigated. We found that metformin could suppress the N6-methyladenosine (m<sup>6</sup>A) level in breast cancer cells significantly. The latter has an essential role in breast cancer progression and is newly considered as a therapeutic target. In this study, we measured the m<sup>6</sup>A level by m<sup>6</sup>A colorimetric analysis and dot blot assay. We then performed qRT-PCR, western blot, MeRIP, dual-luciferase reporter assay, and others to explore the m<sup>6</sup>A-dependent pathway associated with metformin. In vivo effect of metformin was investigated using a mouse tumorigenicity model. In addition, breast cancer and normal tiss","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Jan","modification":"2025-05-31T23:35:04.186Z","creation":"2025-05-31T23:35:04.186Z"},"accession":"S-EPMC7801402","cross_references":{"pubmed":["33431790"],"doi":["10.1038/s41389-020-00290-y"]}}