{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Iriguchi S"],"funding":["Japan Agency for Medical Research and Development","Ministry of Education, Culture, Sports, Science and Technology"],"pagination":["430"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7814014"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12(1)"],"pubmed_abstract":["Clinical successes demonstrated by chimeric antigen receptor T-cell immunotherapy have facilitated further development of T-cell immunotherapy against wide variety of diseases. One approach is the development of \"off-the-shelf\" T-cell sources. Technologies to generate T-cells from pluripotent stem cells (PSCs) may offer platforms to produce \"off-the-shelf\" and synthetic allogeneic T-cells. However, low differentiation efficiency and poor scalability of current methods may compromise their utilities. Here we show improved differentiation efficiency of T-cells from induced PSCs (iPSCs) derived from an antigen-specific cytotoxic T-cell clone, or from T-cell receptor (TCR)-transduced iPSCs, as starting materials. We additionally describe feeder-free differentiation culture systems that span fr"],"journal":["Nature communications"],"pubmed_title":["A clinically applicable and scalable method to regenerate T-cells from iPSCs for off-the-shelf T-cell immunotherapy."],"pmcid":["PMC7814014"],"funding_grant_id":["15J05263","15H04655"],"pubmed_authors":["Yasui Y","Minagawa A","Kassai Y","Arima S","Yanagawa N","Ueda T","Yasukawa M","Kaneko S","Sato T","Baba Y","Kunitomo M","Nakatsura T","Mishima Y","Kawai Y","Shinohara T","Takiguchi M","Hayashi A","Iriguchi S","Miyake Y","Nakayama K"],"additional_accession":[]},"is_claimable":false,"name":"A clinically applicable and scalable method to regenerate T-cells from iPSCs for off-the-shelf T-cell immunotherapy.","description":"Clinical successes demonstrated by chimeric antigen receptor T-cell immunotherapy have facilitated further development of T-cell immunotherapy against wide variety of diseases. One approach is the development of \"off-the-shelf\" T-cell sources. Technologies to generate T-cells from pluripotent stem cells (PSCs) may offer platforms to produce \"off-the-shelf\" and synthetic allogeneic T-cells. However, low differentiation efficiency and poor scalability of current methods may compromise their utilities. Here we show improved differentiation efficiency of T-cells from induced PSCs (iPSCs) derived from an antigen-specific cytotoxic T-cell clone, or from T-cell receptor (TCR)-transduced iPSCs, as starting materials. We additionally describe feeder-free differentiation culture systems that span fr","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Jan","modification":"2025-04-25T17:46:33.331Z","creation":"2021-02-21T01:51:53Z"},"accession":"S-EPMC7814014","cross_references":{"pubmed":["33462228"],"doi":["10.1038/s41467-020-20658-3"]}}