biostudies-literatureIriguchi SJapan Agency for Medical Research and DevelopmentMinistry of Education, Culture, Sports, Science and Technology430https://www.ebi.ac.uk/biostudies/studies/S-EPMC7814014biostudies-literatureUnknown12(1)Clinical successes demonstrated by chimeric antigen receptor T-cell immunotherapy have facilitated further development of T-cell immunotherapy against wide variety of diseases. One approach is the development of "off-the-shelf" T-cell sources. Technologies to generate T-cells from pluripotent stem cells (PSCs) may offer platforms to produce "off-the-shelf" and synthetic allogeneic T-cells. However, low differentiation efficiency and poor scalability of current methods may compromise their utilities. Here we show improved differentiation efficiency of T-cells from induced PSCs (iPSCs) derived from an antigen-specific cytotoxic T-cell clone, or from T-cell receptor (TCR)-transduced iPSCs, as starting materials. We additionally describe feeder-free differentiation culture systems that span frNature communicationsA clinically applicable and scalable method to regenerate T-cells from iPSCs for off-the-shelf T-cell immunotherapy.PMC781401415J0526315H04655Yasui YMinagawa AKassai YArima SYanagawa NUeda TYasukawa MKaneko SSato TBaba YKunitomo MNakatsura TMishima YKawai YShinohara TTakiguchi MHayashi AIriguchi SMiyake YNakayama KfalseA clinically applicable and scalable method to regenerate T-cells from iPSCs for off-the-shelf T-cell immunotherapy.Clinical successes demonstrated by chimeric antigen receptor T-cell immunotherapy have facilitated further development of T-cell immunotherapy against wide variety of diseases. One approach is the development of "off-the-shelf" T-cell sources. Technologies to generate T-cells from pluripotent stem cells (PSCs) may offer platforms to produce "off-the-shelf" and synthetic allogeneic T-cells. However, low differentiation efficiency and poor scalability of current methods may compromise their utilities. Here we show improved differentiation efficiency of T-cells from induced PSCs (iPSCs) derived from an antigen-specific cytotoxic T-cell clone, or from T-cell receptor (TCR)-transduced iPSCs, as starting materials. We additionally describe feeder-free differentiation culture systems that span fr2021-01-01T00:00:00Z2021 Jan2025-04-25T17:46:33.331Z2021-02-21T01:51:53ZS-EPMC78140143346222810.1038/s41467-020-20658-3