biostudies-literatureDuellberg CHuman Frontier Science ProgramAustrian Science Fund FWFe2010054118https://www.ebi.ac.uk/biostudies/studies/S-EPMC7817218biostudies-literatureUnknown118(1)The differentiation of cells depends on a precise control of their internal organization, which is the result of a complex dynamic interplay between the cytoskeleton, molecular motors, signaling molecules, and membranes. For example, in the developing neuron, the protein ADAP1 (ADP-ribosylation factor GTPase-activating protein [ArfGAP] with dual pleckstrin homology [PH] domains 1) has been suggested to control dendrite branching by regulating the small GTPase ARF6. Together with the motor protein KIF13B, ADAP1 is also thought to mediate delivery of the second messenger phosphatidylinositol (3,4,5)-trisphosphate (PIP<sub>3</sub>) to the axon tip, thus contributing to PIP<sub>3</sub> polarity. However, what defines the function of ADAP1 and how its different roles are coordinated are still nProceedings of the National Academy of Sciences of the United States of AmericaIn vitro reconstitution reveals phosphoinositides as cargo-release factors and activators of the ARF6 GAP ADAP1.PMC7817218W 1250YIG RGY0083/2016Duellberg CLoibl KCanigova NAuer ALoose MfalseIn vitro reconstitution reveals phosphoinositides as cargo-release factors and activators of the ARF6 GAP ADAP1.The differentiation of cells depends on a precise control of their internal organization, which is the result of a complex dynamic interplay between the cytoskeleton, molecular motors, signaling molecules, and membranes. For example, in the developing neuron, the protein ADAP1 (ADP-ribosylation factor GTPase-activating protein [ArfGAP] with dual pleckstrin homology [PH] domains 1) has been suggested to control dendrite branching by regulating the small GTPase ARF6. Together with the motor protein KIF13B, ADAP1 is also thought to mediate delivery of the second messenger phosphatidylinositol (3,4,5)-trisphosphate (PIP<sub>3</sub>) to the axon tip, thus contributing to PIP<sub>3</sub> polarity. However, what defines the function of ADAP1 and how its different roles are coordinated are still n2021-01-01T00:00:00Z2021 Jan2026-05-07T16:57:50.955Z2022-02-10T16:39:36.762ZS-EPMC78172183344315310.1073/pnas.2010054118