{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Stepnov AA"],"funding":["Centre for Digital Life Norway"],"pagination":["28"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7818938"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["14(1)"],"pubmed_abstract":["<h4>Background</h4>Lytic polysaccharide monooxygenases (LPMOs) are monocopper enzymes that catalyze oxidative depolymerization of industrially relevant crystalline polysaccharides, such as cellulose, in a reaction that depends on an electron donor and O<sub>2</sub> or H<sub>2</sub>O<sub>2</sub>. While it is well known that LPMOs can utilize a wide variety of electron donors, the variation in reported efficiencies of various LPMO-reductant combinations remains largely unexplained.<h4>Results</h4>In this study, we describe a novel two-domain cellulose-active family AA10 LPMO from a marine actinomycete, which we have used to look more closely at the effects of the reductant and copper ions on the LPMO reaction. Our results show that ascorbate-driven LPMO reactions are extremely sensitive to v"],"journal":["Biotechnology for biofuels"],"pubmed_title":["Unraveling the roles of the reductant and free copper ions in LPMO kinetics."],"pmcid":["PMC7818938"],"funding_grant_id":["269408"],"pubmed_authors":["Wentzel A","Rohr AK","Eijsink VGH","Stepnov AA","Nguyen GS","Forsberg Z","Sorlie M"],"additional_accession":[]},"is_claimable":false,"name":"Unraveling the roles of the reductant and free copper ions in LPMO kinetics.","description":"<h4>Background</h4>Lytic polysaccharide monooxygenases (LPMOs) are monocopper enzymes that catalyze oxidative depolymerization of industrially relevant crystalline polysaccharides, such as cellulose, in a reaction that depends on an electron donor and O<sub>2</sub> or H<sub>2</sub>O<sub>2</sub>. While it is well known that LPMOs can utilize a wide variety of electron donors, the variation in reported efficiencies of various LPMO-reductant combinations remains largely unexplained.<h4>Results</h4>In this study, we describe a novel two-domain cellulose-active family AA10 LPMO from a marine actinomycete, which we have used to look more closely at the effects of the reductant and copper ions on the LPMO reaction. Our results show that ascorbate-driven LPMO reactions are extremely sensitive to v","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Jan","modification":"2026-04-29T20:20:03.763Z","creation":"2021-02-21T01:07:19Z"},"accession":"S-EPMC7818938","cross_references":{"pubmed":["33478537"],"doi":["10.1186/s13068-021-01879-0"]}}