biostudies-literatureStepnov AACentre for Digital Life Norway28https://www.ebi.ac.uk/biostudies/studies/S-EPMC7818938biostudies-literatureUnknown14(1)<h4>Background</h4>Lytic polysaccharide monooxygenases (LPMOs) are monocopper enzymes that catalyze oxidative depolymerization of industrially relevant crystalline polysaccharides, such as cellulose, in a reaction that depends on an electron donor and O₂ or H₂O₂. While it is well known that LPMOs can utilize a wide variety of electron donors, the variation in reported efficiencies of various LPMO-reductant combinations remains largely unexplained.<h4>Results</h4>In this study, we describe a novel two-domain cellulose-active family AA10 LPMO from a marine actinomycete, which we have used to look more closely at the effects of the reductant and copper ions on the LPMO reaction. Our results show that ascorbate-driven LPMO reactions are extremely sensitive to vBiotechnology for biofuelsUnraveling the roles of the reductant and free copper ions in LPMO kinetics.PMC7818938269408Wentzel ARohr AKEijsink VGHStepnov AANguyen GSForsberg ZSorlie MfalseUnraveling the roles of the reductant and free copper ions in LPMO kinetics.<h4>Background</h4>Lytic polysaccharide monooxygenases (LPMOs) are monocopper enzymes that catalyze oxidative depolymerization of industrially relevant crystalline polysaccharides, such as cellulose, in a reaction that depends on an electron donor and O₂ or H₂O₂. While it is well known that LPMOs can utilize a wide variety of electron donors, the variation in reported efficiencies of various LPMO-reductant combinations remains largely unexplained.<h4>Results</h4>In this study, we describe a novel two-domain cellulose-active family AA10 LPMO from a marine actinomycete, which we have used to look more closely at the effects of the reductant and copper ions on the LPMO reaction. Our results show that ascorbate-driven LPMO reactions are extremely sensitive to v2021-01-01T00:00:00Z2021 Jan2026-04-29T20:20:03.763Z2021-02-21T01:07:19ZS-EPMC78189383347853710.1186/s13068-021-01879-0