{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Bardet C"],"funding":["Association Nationale de la Recherche et de la Technologie"],"pagination":["1291-1301"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7826153"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["40(6)"],"pubmed_abstract":["Rapid and reliable pathogen identification is compulsory to confirm ventilator-associated pneumonia (VAP) in order to initiate appropriate antibiotic treatment. In the present proof of concept, the effectiveness of rapid microorganism identification with a targeted bottom-up proteomics approach was investigated in endotracheal aspirate (ETA) samples of VAP patients. To do so, a prototype selected-reaction monitoring (SRM)-based assay was developed on a triple quadrupole mass spectrometer tracking proteotypic peptide surrogates of bacterial proteomes. Through the concurrent monitoring of 97 species-specific peptides, this preliminary assay was dimensioned to characterize the occurrence of six most frequent bacterial species responsible for over more than 65% of VAP. Assay performance was su"],"journal":["European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology"],"pubmed_title":["Early and specific targeted mass spectrometry-based identification of bacteria in endotracheal aspirates of patients suspected with ventilator-associated pneumonia."],"pmcid":["PMC7826153"],"funding_grant_id":["2011/0456"],"pubmed_authors":["Prudent S","Jestin MA","Yugueros-Marcos J","Lacroix M","Fortin T","Chainier D","Duchiron C","VALIBI group","Vignon P","Clavel M","Charrier JP","Francois B","Vignaud J","Desachy A","Le Brun C","Bardet C","Ploy MC","Sommabere A","Garandeau C","Pichon N","Mercier E","Droual R","Barraud O","Rodrigue M","Lemoine J","Mattei M"],"additional_accession":[]},"is_claimable":false,"name":"Early and specific targeted mass spectrometry-based identification of bacteria in endotracheal aspirates of patients suspected with ventilator-associated pneumonia.","description":"Rapid and reliable pathogen identification is compulsory to confirm ventilator-associated pneumonia (VAP) in order to initiate appropriate antibiotic treatment. In the present proof of concept, the effectiveness of rapid microorganism identification with a targeted bottom-up proteomics approach was investigated in endotracheal aspirate (ETA) samples of VAP patients. To do so, a prototype selected-reaction monitoring (SRM)-based assay was developed on a triple quadrupole mass spectrometer tracking proteotypic peptide surrogates of bacterial proteomes. Through the concurrent monitoring of 97 species-specific peptides, this preliminary assay was dimensioned to characterize the occurrence of six most frequent bacterial species responsible for over more than 65% of VAP. Assay performance was su","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Jun","modification":"2026-04-28T21:40:27.984Z","creation":"2021-02-21T04:09:19Z"},"accession":"S-EPMC7826153","cross_references":{"pubmed":["33486654"],"doi":["10.1007/s10096-020-04132-y"]}}