biostudies-literatureBardet CAssociation Nationale de la Recherche et de la Technologie1291-1301https://www.ebi.ac.uk/biostudies/studies/S-EPMC7826153biostudies-literatureUnknown40(6)Rapid and reliable pathogen identification is compulsory to confirm ventilator-associated pneumonia (VAP) in order to initiate appropriate antibiotic treatment. In the present proof of concept, the effectiveness of rapid microorganism identification with a targeted bottom-up proteomics approach was investigated in endotracheal aspirate (ETA) samples of VAP patients. To do so, a prototype selected-reaction monitoring (SRM)-based assay was developed on a triple quadrupole mass spectrometer tracking proteotypic peptide surrogates of bacterial proteomes. Through the concurrent monitoring of 97 species-specific peptides, this preliminary assay was dimensioned to characterize the occurrence of six most frequent bacterial species responsible for over more than 65% of VAP. Assay performance was suEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical MicrobiologyEarly and specific targeted mass spectrometry-based identification of bacteria in endotracheal aspirates of patients suspected with ventilator-associated pneumonia.PMC78261532011/0456Prudent SJestin MAYugueros-Marcos JLacroix MFortin TChainier DDuchiron CVALIBI groupVignon PClavel MCharrier JPFrancois BVignaud JDesachy ALe Brun CBardet CPloy MCSommabere AGarandeau CPichon NMercier EDroual RBarraud ORodrigue MLemoine JMattei MfalseEarly and specific targeted mass spectrometry-based identification of bacteria in endotracheal aspirates of patients suspected with ventilator-associated pneumonia.Rapid and reliable pathogen identification is compulsory to confirm ventilator-associated pneumonia (VAP) in order to initiate appropriate antibiotic treatment. In the present proof of concept, the effectiveness of rapid microorganism identification with a targeted bottom-up proteomics approach was investigated in endotracheal aspirate (ETA) samples of VAP patients. To do so, a prototype selected-reaction monitoring (SRM)-based assay was developed on a triple quadrupole mass spectrometer tracking proteotypic peptide surrogates of bacterial proteomes. Through the concurrent monitoring of 97 species-specific peptides, this preliminary assay was dimensioned to characterize the occurrence of six most frequent bacterial species responsible for over more than 65% of VAP. Assay performance was su2021-01-01T00:00:00Z2021 Jun2026-04-28T21:40:27.984Z2021-02-21T04:09:19ZS-EPMC78261533348665410.1007/s10096-020-04132-y