biostudies-literatureUnknown7(1)Pereira HABSFundação de Amparo à Pesquisa do Estado de São PauloFluoride (F) can induce changes in the expression of several liver proteins, most of them localized in the mitochondria and its effect is dose- and time-dependent. This study analyzed the effect of distinct F concentrations and exposure periods on the mitochondrial activity of complex I-III and II-III in the liver. Thirty-six 21-day-old male Wistar rats were divided into 2 groups (n = 18) according to the duration of the treatment (20 or 60 days). They were subdivided into 3 subgroups (n = 6) according to the concentration of F (0 mg/L, 15 mg/L or 50 mg/L). After the experimental periods, the animals were anesthetized, liver mitochondria were isolated and stored for activity analyses. The determination of complexes II-III and I-III was based on the reduction of cytochrome c³⁺ toHeliyone06028https://www.ebi.ac.uk/biostudies/studies/S-EPMC7829207biostudies-literatureIncrease of complex I and reduction of complex II mitochondrial activity are possible adaptive effects provoked by fluoride exposure.PMC7829207Pereira FSPereira HABSAraujo TTDionizio AIano FGTrevizol JSFaria Ximenes VBuzalaf MARfalseIncrease of complex I and reduction of complex II mitochondrial activity are possible adaptive effects provoked by fluoride exposure.Fluoride (F) can induce changes in the expression of several liver proteins, most of them localized in the mitochondria and its effect is dose- and time-dependent. This study analyzed the effect of distinct F concentrations and exposure periods on the mitochondrial activity of complex I-III and II-III in the liver. Thirty-six 21-day-old male Wistar rats were divided into 2 groups (n = 18) according to the duration of the treatment (20 or 60 days). They were subdivided into 3 subgroups (n = 6) according to the concentration of F (0 mg/L, 15 mg/L or 50 mg/L). After the experimental periods, the animals were anesthetized, liver mitochondria were isolated and stored for activity analyses. The determination of complexes II-III and I-III was based on the reduction of cytochrome c³⁺ to2021-01-01T00:00:00Z2021 Jan2026-04-28T22:53:39.896Z2024-11-13T03:59:09.41ZS-EPMC78292073353264710.1016/j.heliyon.2021.e06028