biostudies-literatureOh KENational Research Foundation of Korea grant funded by the Korean Government446https://www.ebi.ac.uk/biostudies/studies/S-EPMC7830106biostudies-literatureUnknown26(2)<i>Vitis amurensis</i> roots have been reported to have the potential for skin whitening through the evaluation of melanogenesis and tyrosinase inhibitory activities. In this study, <i>V. amurensis</i> roots were utilized to quickly select whitening ingredients using LC-Q-TOF-MS coupled with tyrosinase inhibitory assay, and to optimize the extraction process for use as a skin whitening functional material by response surface methodology. Results showed that <i>V. amurensis</i> roots exhibited tyrosinase inhibitory effects by two stilbene oligomers, ε-viniferin (<b>1</b>) and vitisin B (<b>2</b>), as predicted by LC-Q-TOF-MS coupled with bioassay. The optimal extraction conditions (methanol concentration 66%, solvent volume 140 mL, and extraction time 100 min) for skin whitening ingredientsMolecules (Basel, Switzerland)Characterization and Optimization of the Tyrosinase Inhibitory Activity of <i>Vitis amurensis</i> Root Using LC-Q-TOF-MS Coupled with a Bioassay and Response Surface Methodology.PMC7830106NRF-2016R1A6A1A03011325, NRF-2017R1A2B4003403 and NRF-2019R1A6A1A03031807Oh KELee MKPark BLee KYShin HfalseCharacterization and Optimization of the Tyrosinase Inhibitory Activity of <i>Vitis amurensis</i> Root Using LC-Q-TOF-MS Coupled with a Bioassay and Response Surface Methodology.<i>Vitis amurensis</i> roots have been reported to have the potential for skin whitening through the evaluation of melanogenesis and tyrosinase inhibitory activities. In this study, <i>V. amurensis</i> roots were utilized to quickly select whitening ingredients using LC-Q-TOF-MS coupled with tyrosinase inhibitory assay, and to optimize the extraction process for use as a skin whitening functional material by response surface methodology. Results showed that <i>V. amurensis</i> roots exhibited tyrosinase inhibitory effects by two stilbene oligomers, ε-viniferin (<b>1</b>) and vitisin B (<b>2</b>), as predicted by LC-Q-TOF-MS coupled with bioassay. The optimal extraction conditions (methanol concentration 66%, solvent volume 140 mL, and extraction time 100 min) for skin whitening ingredients2021-01-01T00:00:00Z2021 Jan2026-05-04T23:51:00.235Z2025-06-01T03:56:40.658ZS-EPMC78301063346701110.3390/molecules26020446