{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Stahl-Rommel S"],"funding":["National Aeronautics and Space Administration","NHGRI NIH HHS"],"pagination":["106"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7830261"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12(1)"],"pubmed_abstract":["For the past two decades, microbial monitoring of the International Space Station (ISS) has relied on culture-dependent methods that require return to Earth for analysis. This has a number of limitations, with the most significant being bias towards the detection of culturable organisms and the inherent delay between sample collection and ground-based analysis. In recent years, portable and easy-to-use molecular-based tools, such as Oxford Nanopore Technologies' MinION™ sequencer and miniPCR bio's miniPCR™ thermal cycler, have been validated onboard the ISS. Here, we report on the development, validation, and implementation of a swab-to-sequencer method that provides a culture-independent solution to real-time microbial profiling onboard the ISS. Method development focused on analysis of s"],"journal":["Genes"],"pubmed_title":["Real-Time Culture-Independent Microbial Profiling Onboard the International Space Station Using Nanopore Sequencing."],"pmcid":["PMC7830261"],"funding_grant_id":["ISS Vehicle Office; Office of the Chief Technologist; Advanced Exploration System Life Support Systems","R01 HG010485"],"pubmed_authors":["Turner DJ","Paten B","Stahl-Rommel S","Jain M","Nguyen HN","Aunon-Chancellor SM","Arnold RR","Burton AS","Stoddart D","Akeson M","Sharp GM","John KK","Castro-Wallace SL","Juul S","Castro CL"],"additional_accession":[]},"is_claimable":false,"name":"Real-Time Culture-Independent Microbial Profiling Onboard the International Space Station Using Nanopore Sequencing.","description":"For the past two decades, microbial monitoring of the International Space Station (ISS) has relied on culture-dependent methods that require return to Earth for analysis. This has a number of limitations, with the most significant being bias towards the detection of culturable organisms and the inherent delay between sample collection and ground-based analysis. In recent years, portable and easy-to-use molecular-based tools, such as Oxford Nanopore Technologies' MinION™ sequencer and miniPCR bio's miniPCR™ thermal cycler, have been validated onboard the ISS. Here, we report on the development, validation, and implementation of a swab-to-sequencer method that provides a culture-independent solution to real-time microbial profiling onboard the ISS. Method development focused on analysis of s","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Jan","modification":"2026-04-29T21:28:41.631Z","creation":"2025-04-04T10:55:59.817Z"},"accession":"S-EPMC7830261","cross_references":{"pubmed":["33467183"],"doi":["10.3390/genes12010106"]}}