biostudies-literatureZhu ZNational Natural Science Foundation of China603513https://www.ebi.ac.uk/biostudies/studies/S-EPMC7835968biostudies-literatureUnknown8The stressed cultivations are widely used in microalgae R&D for the biofuel production with the repress on growth to a certain degree, which limits the overall productivity. The balance between the growth and energy storage compounds accumulation is a target needing the combination of both strain selection or construction and culture optimization. Here, an engineered strain of <i>Chlamydomonas reinhardtii</i>, in which the chloroplast type glyceraldehyde-3-phosphate dehydrogenase (cGAPDH) was overexpressed and named as P3-GAPDH, was cultured on the Algal Station platform. Compared with wild type (WT), <i>C. reinhardtii</i> CC137c, in Tris-acetate-phosphate (TAP) medium, the highest density of WT and P3-GAPDH were 1.23 ± 0.13 and 1.74 ± 0.09 g L^-1 within 96 h, and the maximum bioFrontiers in bioengineering and biotechnologyA Carbon Fixation Enhanced <i>Chlamydomonas reinhardtii</i> Strain for Achieving the Double-Win Between Growth and Biofuel Production Under Non-stressed Conditions.PMC78359682187828531771914Tian JLi XZhu ZCao HCao XRong JfalseA Carbon Fixation Enhanced <i>Chlamydomonas reinhardtii</i> Strain for Achieving the Double-Win Between Growth and Biofuel Production Under Non-stressed Conditions.The stressed cultivations are widely used in microalgae R&D for the biofuel production with the repress on growth to a certain degree, which limits the overall productivity. The balance between the growth and energy storage compounds accumulation is a target needing the combination of both strain selection or construction and culture optimization. Here, an engineered strain of <i>Chlamydomonas reinhardtii</i>, in which the chloroplast type glyceraldehyde-3-phosphate dehydrogenase (cGAPDH) was overexpressed and named as P3-GAPDH, was cultured on the Algal Station platform. Compared with wild type (WT), <i>C. reinhardtii</i> CC137c, in Tris-acetate-phosphate (TAP) medium, the highest density of WT and P3-GAPDH were 1.23 ± 0.13 and 1.74 ± 0.09 g L^-1 within 96 h, and the maximum bio2020-01-01T00:00:00Z20202025-04-18T20:44:12.075Z2021-02-21T04:33:58ZS-EPMC78359683351110410.3389/fbioe.2020.603513