{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["7"],"submitter":["Joshi B"],"funding":["Universitetet i Tromsø"],"pubmed_abstract":["Bacterial extracellular vesicles (EVs) have a vital role in bacterial pathogenesis. However, to date, the small RNA-cargo of EVs released by the opportunistic pathogen <i>Staphylococcus aureus</i> has not been characterized. Here, we shed light on the association of small RNAs with EVs secreted by <i>S. aureus</i> MSSA476 cultured in iron-depleted bacteriologic media supplemented with a subinhibitory dosage of vancomycin to mimic infection condition. Confocal microscopy analysis on intact RNase-treated EVs indicated that RNA is associated with EV particles. Transcriptomic followed by bioinformatics analysis of EV-associated RNA revealed the presence of potential gene regulatory small RNAs and high levels of tRNAs. Among the EV-associated enriched small RNAs were SsrA, RsaC and RNAIII. Our "],"journal":["Frontiers in molecular biosciences"],"pagination":["566207"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7838569"],"repository":["biostudies-literature"],"pubmed_title":["Transcriptome Profiling of <i>Staphylococcus aureus</i> Associated Extracellular Vesicles Reveals Presence of Small RNA-Cargo."],"pmcid":["PMC7838569"],"pubmed_authors":["Wai SN","Nadeem A","Askarian F","Singh B","Hegstad K","Joshi B","Johannessen M"],"additional_accession":[]},"is_claimable":false,"name":"Transcriptome Profiling of <i>Staphylococcus aureus</i> Associated Extracellular Vesicles Reveals Presence of Small RNA-Cargo.","description":"Bacterial extracellular vesicles (EVs) have a vital role in bacterial pathogenesis. However, to date, the small RNA-cargo of EVs released by the opportunistic pathogen <i>Staphylococcus aureus</i> has not been characterized. Here, we shed light on the association of small RNAs with EVs secreted by <i>S. aureus</i> MSSA476 cultured in iron-depleted bacteriologic media supplemented with a subinhibitory dosage of vancomycin to mimic infection condition. Confocal microscopy analysis on intact RNase-treated EVs indicated that RNA is associated with EV particles. Transcriptomic followed by bioinformatics analysis of EV-associated RNA revealed the presence of potential gene regulatory small RNAs and high levels of tRNAs. Among the EV-associated enriched small RNAs were SsrA, RsaC and RNAIII. Our ","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020","modification":"2026-04-08T03:40:04.218Z","creation":"2025-04-06T18:17:12.724Z"},"accession":"S-EPMC7838569","cross_references":{"pubmed":["33521050"],"doi":["10.3389/fmolb.2020.566207"]}}