biostudies-literatureUnknown7Joshi BUniversitetet i TromsøBacterial extracellular vesicles (EVs) have a vital role in bacterial pathogenesis. However, to date, the small RNA-cargo of EVs released by the opportunistic pathogen <i>Staphylococcus aureus</i> has not been characterized. Here, we shed light on the association of small RNAs with EVs secreted by <i>S. aureus</i> MSSA476 cultured in iron-depleted bacteriologic media supplemented with a subinhibitory dosage of vancomycin to mimic infection condition. Confocal microscopy analysis on intact RNase-treated EVs indicated that RNA is associated with EV particles. Transcriptomic followed by bioinformatics analysis of EV-associated RNA revealed the presence of potential gene regulatory small RNAs and high levels of tRNAs. Among the EV-associated enriched small RNAs were SsrA, RsaC and RNAIII. Our Frontiers in molecular biosciences566207https://www.ebi.ac.uk/biostudies/studies/S-EPMC7838569biostudies-literatureTranscriptome Profiling of <i>Staphylococcus aureus</i> Associated Extracellular Vesicles Reveals Presence of Small RNA-Cargo.PMC7838569Wai SNNadeem AAskarian FSingh BHegstad KJoshi BJohannessen MfalseTranscriptome Profiling of <i>Staphylococcus aureus</i> Associated Extracellular Vesicles Reveals Presence of Small RNA-Cargo.Bacterial extracellular vesicles (EVs) have a vital role in bacterial pathogenesis. However, to date, the small RNA-cargo of EVs released by the opportunistic pathogen <i>Staphylococcus aureus</i> has not been characterized. Here, we shed light on the association of small RNAs with EVs secreted by <i>S. aureus</i> MSSA476 cultured in iron-depleted bacteriologic media supplemented with a subinhibitory dosage of vancomycin to mimic infection condition. Confocal microscopy analysis on intact RNase-treated EVs indicated that RNA is associated with EV particles. Transcriptomic followed by bioinformatics analysis of EV-associated RNA revealed the presence of potential gene regulatory small RNAs and high levels of tRNAs. Among the EV-associated enriched small RNAs were SsrA, RsaC and RNAIII. Our 2020-01-01T00:00:00Z20202026-04-08T03:40:04.218Z2025-04-06T18:17:12.724ZS-EPMC78385693352105010.3389/fmolb.2020.566207