biostudies-literatureKoenen EJMSwiss National Science FoundationUniversität Zürich1710-1735https://www.ebi.ac.uk/biostudies/studies/S-EPMC7839790biostudies-literatureUnknown107(12)<h4>Premise</h4>Targeted enrichment methods facilitate sequencing of hundreds of nuclear loci to enhance phylogenetic resolution and elucidate why some parts of the "tree of life" are difficult (if not impossible) to resolve. The mimosoid legumes are a prominent pantropical clade of ~3300 species of woody angiosperms for which previous phylogenies have shown extensive lack of resolution, especially among the species-rich and taxonomically challenging ingoids.<h4>Methods</h4>We generated transcriptomes to select low-copy nuclear genes, enrich these via hybrid capture for representative species of most mimosoid genera, and analyze the resulting data using de novo assembly and various phylogenomic tools for species tree inference. We also evaluate gene tree support and conflict for key internAmerican journal of botanyHybrid capture of 964 nuclear genes resolves evolutionary relationships in the mimosoid legumes and reveals the polytomous origins of a large pantropical radiation.PMC7839790135522Kidner CHughes CEde Souza ERBrown GKKoenen EJMIganci JRNicholls JALewis GPLuckow MSimon MFPennington RTde Queiroz LPfalseHybrid capture of 964 nuclear genes resolves evolutionary relationships in the mimosoid legumes and reveals the polytomous origins of a large pantropical radiation.<h4>Premise</h4>Targeted enrichment methods facilitate sequencing of hundreds of nuclear loci to enhance phylogenetic resolution and elucidate why some parts of the "tree of life" are difficult (if not impossible) to resolve. The mimosoid legumes are a prominent pantropical clade of ~3300 species of woody angiosperms for which previous phylogenies have shown extensive lack of resolution, especially among the species-rich and taxonomically challenging ingoids.<h4>Methods</h4>We generated transcriptomes to select low-copy nuclear genes, enrich these via hybrid capture for representative species of most mimosoid genera, and analyze the resulting data using de novo assembly and various phylogenomic tools for species tree inference. We also evaluate gene tree support and conflict for key intern2020-01-01T00:00:00Z2020 Dec2026-05-02T19:22:20.239Z2021-02-21T06:32:15ZS-EPMC78397903325342310.1002/ajb2.1568