{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Keeble AH"],"funding":["Biotechnology and Biological Sciences Research Council"],"pagination":["7281-7291"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7844731"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["11(28)"],"pubmed_abstract":["Proteins span an extraordinary range of shapes, sizes and functionalities. Therefore generic approaches are needed to overcome this diversity and stream-line protein analysis or application. Here we review SpyTag technology, now used in hundreds of publications or patents, and its potential for detecting and controlling protein behaviour. SpyTag forms a spontaneous and irreversible isopeptide bond upon binding its protein partner SpyCatcher, where both parts are genetically-encoded. New variants of this pair allow reaction at a rate approaching the diffusion limit, while reversible versions allow purification of SpyTagged proteins or tuned dynamic interaction inside cells. Anchoring of SpyTag-linked proteins has been established to diverse nanoparticles or surfaces, including gold, graphen"],"journal":["Chemical science"],"pubmed_title":["Power to the protein: enhancing and combining activities using the Spy toolbox."],"pmcid":["PMC7844731"],"funding_grant_id":["BB/S007369/1"],"pubmed_authors":["Keeble AH","Howarth M"],"additional_accession":[]},"is_claimable":false,"name":"Power to the protein: enhancing and combining activities using the Spy toolbox.","description":"Proteins span an extraordinary range of shapes, sizes and functionalities. Therefore generic approaches are needed to overcome this diversity and stream-line protein analysis or application. Here we review SpyTag technology, now used in hundreds of publications or patents, and its potential for detecting and controlling protein behaviour. SpyTag forms a spontaneous and irreversible isopeptide bond upon binding its protein partner SpyCatcher, where both parts are genetically-encoded. New variants of this pair allow reaction at a rate approaching the diffusion limit, while reversible versions allow purification of SpyTagged proteins or tuned dynamic interaction inside cells. Anchoring of SpyTag-linked proteins has been established to diverse nanoparticles or surfaces, including gold, graphen","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Jul","modification":"2025-04-05T12:02:39.742Z","creation":"2021-02-21T08:32:22Z"},"accession":"S-EPMC7844731","cross_references":{"pubmed":["33552459"],"doi":["10.1039/d0sc01878c"]}}