{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Guilhamon P"],"funding":["NSERC","Jessica's Footprint","The Bresler Family","Princess Margaret Cancer Foundation","Hopeful Minds","Terry Fox Research Institute","Canadian Cancer Society","Stand Up To Cancer Canada, Genome Canada, CIHR, OICR, AACR","Alliance for Cancer Gene Therapy","Hospital for Sick Children","CIHR"],"pagination":["e64090"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7847307"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["10"],"pubmed_abstract":["Chromatin accessibility discriminates stem from mature cell populations, enabling the identification of primitive stem-like cells in primary tumors, such as glioblastoma (GBM) where self-renewing cells driving cancer progression and recurrence are prime targets for therapeutic intervention. We show, using single-cell chromatin accessibility, that primary human GBMs harbor a heterogeneous self-renewing population whose diversity is captured in patient-derived glioblastoma stem cells (GSCs). In-depth characterization of chromatin accessibility in GSCs identifies three GSC states: Reactive, Constructive, and Invasive, each governed by uniquely essential transcription factors and present within GBMs in varying proportions. Orthotopic xenografts reveal that GSC states associate with survival, a"],"journal":["eLife"],"pubmed_title":["Single-cell chromatin accessibility profiling of glioblastoma identifies an invasive cancer stem cell population associated with lower survival."],"pmcid":["PMC7847307"],"funding_grant_id":["MFE 338954","SU2C-AACR-DT-19-15","TGH-158221"],"pubmed_authors":["Guilhamon P","Dirks PB","Ellestad KI","Hassam R","Rajakulendran N","Hao X","Singhal D","Shoichet MS","Rastegar N","Lupien M","Smith LJ","Chesnelong C","Weiss S","Cavalli FM","Haibe-Kains B","Taylor MD","MacLeod G","Chan JA","Gallo M","Whetstone H","Kushida MM","Nadorp B","Arlidge C","Nikolic A","Luchman HA","Coutinho FJ","Angers S","Madani Tonekaboni SA"],"additional_accession":[]},"is_claimable":false,"name":"Single-cell chromatin accessibility profiling of glioblastoma identifies an invasive cancer stem cell population associated with lower survival.","description":"Chromatin accessibility discriminates stem from mature cell populations, enabling the identification of primitive stem-like cells in primary tumors, such as glioblastoma (GBM) where self-renewing cells driving cancer progression and recurrence are prime targets for therapeutic intervention. We show, using single-cell chromatin accessibility, that primary human GBMs harbor a heterogeneous self-renewing population whose diversity is captured in patient-derived glioblastoma stem cells (GSCs). In-depth characterization of chromatin accessibility in GSCs identifies three GSC states: Reactive, Constructive, and Invasive, each governed by uniquely essential transcription factors and present within GBMs in varying proportions. Orthotopic xenografts reveal that GSC states associate with survival, a","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Jan","modification":"2025-04-26T02:35:57.06Z","creation":"2025-04-06T10:31:01.332Z"},"accession":"S-EPMC7847307","cross_references":{"pubmed":["33427645"],"doi":["10.7554/eLife.64090"]}}