biostudies-literatureHersh JNIA NIH HHSNINDS NIH HHSNational Institutes of HealthCancer Prevention and Research Institute of Texas577455https://www.ebi.ac.uk/biostudies/studies/S-EPMC7856103biostudies-literatureUnknown351We determined that T-cell astrocyte interaction modulates interleukin-10 (IL-10) production from both cell types. The impact of IL-10 on astrocytes was compared to IL-10 generated from T-cell-astrocyte interactions in vitro. We demonstrated that T-cells directly interact with astrocytes to upregulate gene expression and secretion of IL-10, confirmed by elevated STAT3p/STAT3 expression in astrocytes. IL-10 increased astrocytes proliferation. In addition, IL-10 treatment and CD4+ co-culture shifts primary astrocytes toward a more energetic phenotype. These findings indicate that direct interaction of CD4+ T-cells with astrocytes, activated the IL-10 anti-inflammatory pathway, altering astrocyte phenotype, metabolism, and proliferation.Journal of neuroimmunologyModulation of astrocyte phenotype in response to T-cell interaction.PMC7856103T32 AG020494RF1 NS109583R01 NS088596Hersh JPrah JLiu RYang SHWinters AfalseModulation of astrocyte phenotype in response to T-cell interaction.We determined that T-cell astrocyte interaction modulates interleukin-10 (IL-10) production from both cell types. The impact of IL-10 on astrocytes was compared to IL-10 generated from T-cell-astrocyte interactions in vitro. We demonstrated that T-cells directly interact with astrocytes to upregulate gene expression and secretion of IL-10, confirmed by elevated STAT3p/STAT3 expression in astrocytes. IL-10 increased astrocytes proliferation. In addition, IL-10 treatment and CD4+ co-culture shifts primary astrocytes toward a more energetic phenotype. These findings indicate that direct interaction of CD4+ T-cells with astrocytes, activated the IL-10 anti-inflammatory pathway, altering astrocyte phenotype, metabolism, and proliferation.2021-01-01T00:00:00Z2021 Feb2025-04-04T02:25:16.868Z2025-04-04T02:25:16.868ZS-EPMC78561033337067110.1016/j.jneuroim.2020.577455