{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["2(1)"],"submitter":["Becker KA"],"funding":["Bundesministerium für Bildung und Forschung","Deutsche Forschungsgemeinschaft"],"pubmed_abstract":["This protocol enables the testing of drugs against infection of epithelial cells with SARS-CoV-2 (severe acute respiratory syndrome coronavirus-2), using pseudo-typed replication deficient vesicular stomatitis virus particles (pp-VSV) presenting the SARS-CoV-2 spike protein. After treating human volunteers with amitriptyline, an approved antidepressant and inhibitor of the acid sphingomyelinase, freshly isolated nasal epithelial cells were infected <i>ex vivo</i> and infection levels were quantified. This protocol offers the possibility to rapidly test the efficacy of potential drugs in the fight against COVID-19. For complete details on the use and execution of this protocol, please refer to Carpinteiro et al. (2020)."],"journal":["STAR protocols"],"pagination":["100356"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7857112"],"repository":["biostudies-literature"],"pubmed_title":["<i>Ex vivo</i> assay to evaluate the efficacy of drugs targeting sphingolipids in preventing SARS-CoV-2 infection of nasal epithelial cells."],"pmcid":["PMC7857112"],"pubmed_authors":["Becker KA","Gulbins E","Carpinteiro A","Hoffmann M","Kornhuber J","Pohlmann S"],"additional_accession":[]},"is_claimable":false,"name":"<i>Ex vivo</i> assay to evaluate the efficacy of drugs targeting sphingolipids in preventing SARS-CoV-2 infection of nasal epithelial cells.","description":"This protocol enables the testing of drugs against infection of epithelial cells with SARS-CoV-2 (severe acute respiratory syndrome coronavirus-2), using pseudo-typed replication deficient vesicular stomatitis virus particles (pp-VSV) presenting the SARS-CoV-2 spike protein. After treating human volunteers with amitriptyline, an approved antidepressant and inhibitor of the acid sphingomyelinase, freshly isolated nasal epithelial cells were infected <i>ex vivo</i> and infection levels were quantified. This protocol offers the possibility to rapidly test the efficacy of potential drugs in the fight against COVID-19. For complete details on the use and execution of this protocol, please refer to Carpinteiro et al. (2020).","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Mar","modification":"2025-06-01T12:18:01.169Z","creation":"2021-02-22T08:09:08Z"},"accession":"S-EPMC7857112","cross_references":{"pubmed":["33558862"],"doi":["10.1016/j.xpro.2021.100356"]}}