biostudies-literatureVasquez MTHHS | NIH | National Institute of General Medical SciencesNIAID NIH HHSBurroughs Wellcome FundCystic Fibrosis FoundationNCI NIH HHSHHS | NIH | National Institute of Allergy and Infectious DiseasesDepartment of Health, Australian Government | National Health and Medical Research CouncilNIGMS NIH HHS17241-17250https://www.ebi.ac.uk/biostudies/studies/S-EPMC7863875biostudies-literatureUnknown295(50)Leukocidin ED (LukED) is a pore-forming toxin produced by <i>Staphylococcus aureus</i>, which lyses host cells and promotes virulence of the bacteria. LukED enables <i>S. aureus</i> to acquire iron by lysing erythrocytes, which depends on targeting the host receptor Duffy antigen receptor for chemokines (DARC). The toxin also targets DARC on the endothelium, contributing to the lethality observed during bloodstream infection in mice. LukED is comprised of two monomers: LukE and LukD. LukE binds to DARC and facilitates hemolysis, but the closely related Panton-Valentine leukocidin S (LukS-PV) does not bind to DARC and is not hemolytic. The interaction of LukE with DARC and the role this plays in hemolysis are incompletely characterized. To determine the domain(s) of LukE that are critical fThe Journal of biological chemistryIdentification of a domain critical for <i>Staphylococcus aureus</i> LukED receptor targeting and lysis of erythrocytes.PMC7863875HHSN272201400019CF31 AI112290R01 AI105129T32 GM007308LACEY19F0R01AI105129F30 AI124606T32 AI007180 and F31 AI1122901013513P30 CA0160871138466R01 AI105129-04S1T32 AI007180Reyes-Robles TTorres VJJennings MPLacey KAVasquez MTLubkin ADay CJfalseIdentification of a domain critical for <i>Staphylococcus aureus</i> LukED receptor targeting and lysis of erythrocytes.Leukocidin ED (LukED) is a pore-forming toxin produced by <i>Staphylococcus aureus</i>, which lyses host cells and promotes virulence of the bacteria. LukED enables <i>S. aureus</i> to acquire iron by lysing erythrocytes, which depends on targeting the host receptor Duffy antigen receptor for chemokines (DARC). The toxin also targets DARC on the endothelium, contributing to the lethality observed during bloodstream infection in mice. LukED is comprised of two monomers: LukE and LukD. LukE binds to DARC and facilitates hemolysis, but the closely related Panton-Valentine leukocidin S (LukS-PV) does not bind to DARC and is not hemolytic. The interaction of LukE with DARC and the role this plays in hemolysis are incompletely characterized. To determine the domain(s) of LukE that are critical f2020-01-01T00:00:00Z2020 Dec2026-05-02T11:43:06.679Z2022-02-11T12:05:14.828ZS-EPMC78638753305121010.1074/jbc.ra120.01575710.1074/jbc.RA120.015757