biostudies-literatureWojcik-Jagla MNarodowe Centrum Nauki1057https://www.ebi.ac.uk/biostudies/studies/S-EPMC7865787biostudies-literatureUnknown22(3)Mechanisms involved in the de-acclimation of herbaceous plants caused by warm periods during winter are poorly understood. This study identifies the genes associated with this mechanism in winter barley. Seedlings of eight accessions (four tolerant and four susceptible to de-acclimation cultivars and advanced breeding lines) were cold acclimated for three weeks and de-acclimated at 12 °C/5 °C (day/night) for one week. We performed differential expression analysis using RNA sequencing. In addition, reverse-transcription quantitative real-time PCR and enzyme activity analyses were used to investigate changes in the expression of selected genes. The number of transcripts with accumulation level changed in opposite directions during acclimation and de-acclimation was much lower than the numberInternational journal of molecular sciencesIdentification of the Genetic Basis of Response to De-Acclimation in Winter Barley.PMC78657872016/21/D/NZ9/01318Daszkowska-Golec AFiust ARapacz MWojcik-Jagla MKopec PfalseIdentification of the Genetic Basis of Response to De-Acclimation in Winter Barley.Mechanisms involved in the de-acclimation of herbaceous plants caused by warm periods during winter are poorly understood. This study identifies the genes associated with this mechanism in winter barley. Seedlings of eight accessions (four tolerant and four susceptible to de-acclimation cultivars and advanced breeding lines) were cold acclimated for three weeks and de-acclimated at 12 °C/5 °C (day/night) for one week. We performed differential expression analysis using RNA sequencing. In addition, reverse-transcription quantitative real-time PCR and enzyme activity analyses were used to investigate changes in the expression of selected genes. The number of transcripts with accumulation level changed in opposite directions during acclimation and de-acclimation was much lower than the number2021-01-01T00:00:00Z2021 Jan2026-04-15T03:16:57.454Z2021-02-21T08:29:56ZS-EPMC78657873349437110.3390/ijms22031057