biostudies-literatureChatterjee SMacquarie UniversityRoyal Adelaide HospitalCancer Institute NSWAustralian Cystic Fibrosis Research Trust516https://www.ebi.ac.uk/biostudies/studies/S-EPMC7867038biostudies-literatureUnknown10(3)Bacteremia-i.e., the presence of pathogens in the blood stream-is associated with long-term morbidity and is a potential precursor condition to life-threatening sepsis. Timely detection of bacteremia is therefore critical to reduce patient mortality, but existing methods lack precision, speed, and sensitivity to effectively stratify bacteremic patients. Herein, we tested the potential of quantitative serum N-glycomics performed using porous graphitized carbon liquid chromatography tandem mass spectrometry to stratify bacteremic patients infected with Escherichia coli (n = 11), Staphylococcus aureus (n = 11), Pseudomonas aeruginosa (n = 5), and Streptococcus viridans (n = 5) from healthy donors (n = 39). In total, 62 N-glycan isomers spanning 41 glycan compositions primarily comprising compJournal of clinical medicineSerum N-Glycomics Stratifies Bacteremic Patients Infected with Different Pathogens.PMC7867038International Macquarie Research Excellence ScholarshipA.R Clarkson ScholarshipMary Overton Early Career FellowshipPostgraduate studentship awardClinical Project GrantMacquarie University Safety Net GrantEarly Career FellowshipTjondro HCKawahara RTorpy DJShaw DRThaysen-Andersen MNenke MAChatterjee SfalseSerum N-Glycomics Stratifies Bacteremic Patients Infected with Different Pathogens.Bacteremia-i.e., the presence of pathogens in the blood stream-is associated with long-term morbidity and is a potential precursor condition to life-threatening sepsis. Timely detection of bacteremia is therefore critical to reduce patient mortality, but existing methods lack precision, speed, and sensitivity to effectively stratify bacteremic patients. Herein, we tested the potential of quantitative serum N-glycomics performed using porous graphitized carbon liquid chromatography tandem mass spectrometry to stratify bacteremic patients infected with Escherichia coli (n = 11), Staphylococcus aureus (n = 11), Pseudomonas aeruginosa (n = 5), and Streptococcus viridans (n = 5) from healthy donors (n = 39). In total, 62 N-glycan isomers spanning 41 glycan compositions primarily comprising comp2021-01-01T00:00:00Z2021 Feb2025-04-04T18:44:17.869Z2021-02-21T07:24:15ZS-EPMC78670383353557110.3390/jcm10030516