{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Earley DF"],"funding":["Swiss National Science Foundation","Horizon 2020 Framework Programme","Krebsliga Schweiz"],"pagination":["764"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7867232"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["26(3)"],"pubmed_abstract":["89Zr-radiolabelled proteins functionalised with desferrioxamine B are a cornerstone of diagnostic positron emission tomography. In the clinical setting, 89Zr-labelled proteins are produced manually. Here, we explore the potential of using a microfluidic photochemical flow reactor to prepare 89Zr-radiolabelled proteins. The light-induced functionalisation and 89Zr-radiolabelling of human serum albumin ([89Zr]ZrDFO-PEG3-Et-azepin-HSA) was achieved by flow photochemistry with a decay-corrected radiochemical yield (RCY) of 31.2 ± 1.3% (n = 3) and radiochemical purity >90%. In comparison, a manual batch photoreactor synthesis produced the same radiotracer in a decay-corrected RCY of 59.6 ± 3.6% (n = 3) with an equivalent RCP > 90%. The results indicate that photoradiolabelling in flow is a feasible platform for the automated production of protein-based 89Zr-radiotracers, but further refinement of the apparatus and optimisation of the method are required before the flow process is competitive with manual reactions."],"journal":["Molecules (Basel, Switzerland)"],"pubmed_title":["Microfluidic Preparation of 89Zr-Radiolabelled Proteins by Flow Photochemistry."],"pmcid":["PMC7867232"],"funding_grant_id":["rant Agreement No: 676904, ERC-StG-2015, NanoSCAN; 101001734, ERC-CoG-2020, PhotoPHARMA","PP00P2_163683 and PP00P2_190093","KLS-4257-08-2017"],"pubmed_authors":["Holland JP","van der Born D","Earley DF","Guillou A","Poot AJ"],"additional_accession":[]},"is_claimable":false,"name":"Microfluidic Preparation of 89Zr-Radiolabelled Proteins by Flow Photochemistry.","description":"89Zr-radiolabelled proteins functionalised with desferrioxamine B are a cornerstone of diagnostic positron emission tomography. In the clinical setting, 89Zr-labelled proteins are produced manually. Here, we explore the potential of using a microfluidic photochemical flow reactor to prepare 89Zr-radiolabelled proteins. The light-induced functionalisation and 89Zr-radiolabelling of human serum albumin ([89Zr]ZrDFO-PEG3-Et-azepin-HSA) was achieved by flow photochemistry with a decay-corrected radiochemical yield (RCY) of 31.2 ± 1.3% (n = 3) and radiochemical purity >90%. In comparison, a manual batch photoreactor synthesis produced the same radiotracer in a decay-corrected RCY of 59.6 ± 3.6% (n = 3) with an equivalent RCP > 90%. The results indicate that photoradiolabelling in flow is a feasible platform for the automated production of protein-based 89Zr-radiotracers, but further refinement of the apparatus and optimisation of the method are required before the flow process is competitive with manual reactions.","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Feb","modification":"2025-04-04T22:04:07.81Z","creation":"2025-04-04T22:04:07.81Z"},"accession":"S-EPMC7867232","cross_references":{"pubmed":["33540712"],"doi":["10.3390/molecules26030764"]}}