biostudies-literatureEarley DFSwiss National Science FoundationHorizon 2020 Framework ProgrammeKrebsliga Schweiz764https://www.ebi.ac.uk/biostudies/studies/S-EPMC7867232biostudies-literatureUnknown26(3)89Zr-radiolabelled proteins functionalised with desferrioxamine B are a cornerstone of diagnostic positron emission tomography. In the clinical setting, 89Zr-labelled proteins are produced manually. Here, we explore the potential of using a microfluidic photochemical flow reactor to prepare 89Zr-radiolabelled proteins. The light-induced functionalisation and 89Zr-radiolabelling of human serum albumin ([89Zr]ZrDFO-PEG3-Et-azepin-HSA) was achieved by flow photochemistry with a decay-corrected radiochemical yield (RCY) of 31.2 ± 1.3% (n = 3) and radiochemical purity >90%. In comparison, a manual batch photoreactor synthesis produced the same radiotracer in a decay-corrected RCY of 59.6 ± 3.6% (n = 3) with an equivalent RCP > 90%. The results indicate that photoradiolabelling in flow is a feasible platform for the automated production of protein-based 89Zr-radiotracers, but further refinement of the apparatus and optimisation of the method are required before the flow process is competitive with manual reactions.Molecules (Basel, Switzerland)Microfluidic Preparation of 89Zr-Radiolabelled Proteins by Flow Photochemistry.PMC7867232rant Agreement No: 676904, ERC-StG-2015, NanoSCAN; 101001734, ERC-CoG-2020, PhotoPHARMAPP00P2_163683 and PP00P2_190093KLS-4257-08-2017Holland JPvan der Born DEarley DFGuillou APoot AJfalseMicrofluidic Preparation of 89Zr-Radiolabelled Proteins by Flow Photochemistry.89Zr-radiolabelled proteins functionalised with desferrioxamine B are a cornerstone of diagnostic positron emission tomography. In the clinical setting, 89Zr-labelled proteins are produced manually. Here, we explore the potential of using a microfluidic photochemical flow reactor to prepare 89Zr-radiolabelled proteins. The light-induced functionalisation and 89Zr-radiolabelling of human serum albumin ([89Zr]ZrDFO-PEG3-Et-azepin-HSA) was achieved by flow photochemistry with a decay-corrected radiochemical yield (RCY) of 31.2 ± 1.3% (n = 3) and radiochemical purity >90%. In comparison, a manual batch photoreactor synthesis produced the same radiotracer in a decay-corrected RCY of 59.6 ± 3.6% (n = 3) with an equivalent RCP > 90%. The results indicate that photoradiolabelling in flow is a feasible platform for the automated production of protein-based 89Zr-radiotracers, but further refinement of the apparatus and optimisation of the method are required before the flow process is competitive with manual reactions.2021-01-01T00:00:00Z2021 Feb2025-04-04T22:04:07.81Z2025-04-04T22:04:07.81ZS-EPMC78672323354071210.3390/molecules26030764