{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ramadan M"],"funding":["National Key Research and Development Program of China"],"pagination":["16"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7869495"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["17(1)"],"pubmed_abstract":["<h4>Background</h4>Upland cotton (Gossypium hirsutum), harboring a complex allotetraploid genome, consists of A and D sub-genomes. Every gene has multiple copies with high sequence similarity that makes genetic, genomic and functional analyses extremely challenging. The recent accessibility of CRISPR/Cas9 tool provides the ability to modify targeted locus efficiently in various complicated plant genomes. However, current cotton transformation method targeting one gene requires a complicated, long and laborious regeneration process. Hence, optimizing strategy that targeting multiple genes is of great value in cotton functional genomics and genetic engineering.<h4>Results</h4>To target multiple genes in a single experiment, 112 plant development-related genes were knocked out via optimized C"],"journal":["Plant methods"],"pubmed_title":["Efficient CRISPR/Cas9 mediated Pooled-sgRNAs assembly accelerates targeting multiple genes related to male sterility in cotton."],"pmcid":["PMC7869495"],"funding_grant_id":["2016YFD0101402"],"pubmed_authors":["Min L","Ramadan M","Alariqi M","Li Y","Liu Z","Jin S","Zhang X","Ma Y","Zhang R"],"additional_accession":[]},"is_claimable":false,"name":"Efficient CRISPR/Cas9 mediated Pooled-sgRNAs assembly accelerates targeting multiple genes related to male sterility in cotton.","description":"<h4>Background</h4>Upland cotton (Gossypium hirsutum), harboring a complex allotetraploid genome, consists of A and D sub-genomes. Every gene has multiple copies with high sequence similarity that makes genetic, genomic and functional analyses extremely challenging. The recent accessibility of CRISPR/Cas9 tool provides the ability to modify targeted locus efficiently in various complicated plant genomes. However, current cotton transformation method targeting one gene requires a complicated, long and laborious regeneration process. Hence, optimizing strategy that targeting multiple genes is of great value in cotton functional genomics and genetic engineering.<h4>Results</h4>To target multiple genes in a single experiment, 112 plant development-related genes were knocked out via optimized C","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Feb","modification":"2025-04-18T15:58:12.194Z","creation":"2025-04-07T02:53:37.688Z"},"accession":"S-EPMC7869495","cross_references":{"pubmed":["33557889"],"doi":["10.1186/s13007-021-00712-x"]}}