biostudies-literatureUnknown38(2)Khan HL<h4>Purpose</h4>Intrafollicular fluid (IFF) melatonin plays a decisive role in maintaining granulosa cells' DNA integrity and protects them against apoptosis. It reduces oxidative stress and improves the oocyte quality with a higher fertilization rate.<h4>Method</h4>This prospective study investigated the antioxidant property of IFF melatonin and its impact on IVF outcome parameters. We also explored the relative expression of five microRNAs (miR-663b, miR-320a, miR-766-3p, miR-132-3p, miR-16-5p) and levels of cell-free DNA (cfDNA) by real-time PCR in unexplained infertile patients. We collected 425 follicular fluid (FF) samples containing mature oocytes from 295 patients undergoing IVF.<h4>Results</h4>Patients were subgrouped based on IFF melatonin concentration (group A ≤ 30 pg/mL, groupJournal of assisted reproduction and genetics443-459https://www.ebi.ac.uk/biostudies/studies/S-EPMC7884535biostudies-literatureMelatonin levels and microRNA (miRNA) relative expression profile in the follicular ambient microenvironment in patients undergoing in vitro fertilization process.PMC7884535Hassan ZAydin HHAbbas SBhatti SQurat-Ul-Ain Zahra STurhan NOKaloglu CKhan YLKhan HLfalseMelatonin levels and microRNA (miRNA) relative expression profile in the follicular ambient microenvironment in patients undergoing in vitro fertilization process.<h4>Purpose</h4>Intrafollicular fluid (IFF) melatonin plays a decisive role in maintaining granulosa cells' DNA integrity and protects them against apoptosis. It reduces oxidative stress and improves the oocyte quality with a higher fertilization rate.<h4>Method</h4>This prospective study investigated the antioxidant property of IFF melatonin and its impact on IVF outcome parameters. We also explored the relative expression of five microRNAs (miR-663b, miR-320a, miR-766-3p, miR-132-3p, miR-16-5p) and levels of cell-free DNA (cfDNA) by real-time PCR in unexplained infertile patients. We collected 425 follicular fluid (FF) samples containing mature oocytes from 295 patients undergoing IVF.<h4>Results</h4>Patients were subgrouped based on IFF melatonin concentration (group A ≤ 30 pg/mL, group2021-01-01T00:00:00Z2021 Feb2025-04-20T03:45:21.566Z2022-02-11T15:55:25.319ZS-EPMC78845353322653110.1007/s10815-020-02010-2