{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["O'Connell LA"],"funding":["Howard Hughes Medical Institute","National Institutes of Health","Harvard University","L&apos;Oreal For Women in Science","NIGMS NIH HHS","National Science Foundation"],"pagination":["jeb230342"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7888741"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["224(Pt 3)"],"pubmed_abstract":["Poison frogs sequester chemical defenses from their diet of leaf litter arthropods for defense against predation. Little is known about the physiological adaptations that confer this unusual bioaccumulation ability. We conducted an alkaloid-feeding experiment with the Diablito poison frog (<i>Oophaga sylvatica</i>) to determine how quickly alkaloids are accumulated and how toxins modify frog physiology using quantitative proteomics. Diablito frogs rapidly accumulated the alkaloid decahydroquinoline within 4 days, and dietary alkaloid exposure altered protein abundance in the intestines, liver and skin. Many proteins that increased in abundance with decahydroquinoline accumulation are plasma glycoproteins, including the complement system and the toxin-binding protein saxiphilin. Other protein classes that change in abundance with decahydroquinoline accumulation are membrane proteins involved in small molecule transport and metabolism. Overall, this work shows that poison frogs can rapidly accumulate alkaloids, which alter carrier protein abundance, initiate an immune response, and alter small molecule transport and metabolism dynamics across tissues."],"journal":["The Journal of experimental biology"],"pubmed_title":["Rapid toxin sequestration modifies poison frog physiology."],"pmcid":["PMC7888741"],"funding_grant_id":["R01 GM132129","IOS-1557684","GM67945","520008146","GM132129","R01 GM067945"],"pubmed_authors":["O'Connell JD","Paulo JA","LS50: Integrated Science Laboratory Course","Trauger SA","Gygi SP","Murray AW","O'Connell LA"],"additional_accession":[]},"is_claimable":false,"name":"Rapid toxin sequestration modifies poison frog physiology.","description":"Poison frogs sequester chemical defenses from their diet of leaf litter arthropods for defense against predation. Little is known about the physiological adaptations that confer this unusual bioaccumulation ability. We conducted an alkaloid-feeding experiment with the Diablito poison frog (<i>Oophaga sylvatica</i>) to determine how quickly alkaloids are accumulated and how toxins modify frog physiology using quantitative proteomics. Diablito frogs rapidly accumulated the alkaloid decahydroquinoline within 4 days, and dietary alkaloid exposure altered protein abundance in the intestines, liver and skin. Many proteins that increased in abundance with decahydroquinoline accumulation are plasma glycoproteins, including the complement system and the toxin-binding protein saxiphilin. Other protein classes that change in abundance with decahydroquinoline accumulation are membrane proteins involved in small molecule transport and metabolism. Overall, this work shows that poison frogs can rapidly accumulate alkaloids, which alter carrier protein abundance, initiate an immune response, and alter small molecule transport and metabolism dynamics across tissues.","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Feb","modification":"2026-04-29T10:08:28.487Z","creation":"2025-02-19T03:53:41.799Z"},"accession":"S-EPMC7888741","cross_references":{"pubmed":["33408255"],"doi":["10.1242/jeb.230342"]}}