biostudies-literatureUnknown44(3)Klijn MEKarlsruher Institut für Technologie (KIT)The protein cloud-point temperature (T_Cloud) is a known representative of protein-protein interaction strength and provides valuable information during the development and characterization of protein-based products, such as biopharmaceutics. A high-throughput low volume T_Cloud detection method was introduced in preceding work, where it was concluded that the extracted value is an apparent T_Cloud (T_Cloud,app). As an understanding of the apparent nature is imperative to facilitate inter-study data comparability, the current work was performed to systematically evaluate the influence of 3 image analysis strategies and 2 experimental parameters (sample volume and cooling rate) on T_Cloud,app detection of lysozyme. Different image analysis sBioprocess and biosystems engineering525-536https://www.ebi.ac.uk/biostudies/studies/S-EPMC7889528biostudies-literatureInfluence of image analysis strategy, cooling rate, and sample volume on apparent protein cloud-point temperature determination.PMC7889528Klijn MEHubbuch JfalseInfluence of image analysis strategy, cooling rate, and sample volume on apparent protein cloud-point temperature determination.The protein cloud-point temperature (T_Cloud) is a known representative of protein-protein interaction strength and provides valuable information during the development and characterization of protein-based products, such as biopharmaceutics. A high-throughput low volume T_Cloud detection method was introduced in preceding work, where it was concluded that the extracted value is an apparent T_Cloud (T_Cloud,app). As an understanding of the apparent nature is imperative to facilitate inter-study data comparability, the current work was performed to systematically evaluate the influence of 3 image analysis strategies and 2 experimental parameters (sample volume and cooling rate) on T_Cloud,app detection of lysozyme. Different image analysis s2021-01-01T00:00:00Z2021 Mar2025-04-04T13:38:24.817Z2021-03-06T08:17:49ZS-EPMC78895283323739910.1007/s00449-020-02465-8