biostudies-literatureGao Jthe National Key R&amp;D Program of ChinaR&amp;D Plan of Guangdong Province Key FieldsModern Agricultural Innovation Team Project of Guangdong ProvinceGuangzhou Scientific Research PlanNational Natural Science Foundation of Chinathe National Key R&D Program of ChinaR&D Plan of Guangdong Province Key FieldsScience and Technology Plan Project of Guangdong Province97https://www.ebi.ac.uk/biostudies/studies/S-EPMC7890976biostudies-literatureUnknown21(1)<h4>Background</h4>Banana is a tropical fruit with a high economic impact worldwide. Cold stress greatly affects the development and production of banana.<h4>Results</h4>In the present study, we investigated the functions of MaMAPK3 and MaICE1 involved in cold tolerance of banana. The effect of RNAi of MaMAPK3 on Dajiao (Musa spp. 'Dajiao'; ABB Group) cold tolerance was evaluated. The leaves of the MaMAPK3 RNAi transgenic plants showed wilting and severe necrotic symptoms, while the wide-type (WT) plants remained normal after cold exposure. RNAi of MaMAPK3 significantly changed the expressions of the cold-responsive genes, and the oxidoreductase activity was significantly changed in WT plants, while no changes in transgenic plants were observed. MaICE1 interacted with MaMAPK3, and the exprBMC plant biologyMaMAPK3-MaICE1-MaPOD P7 pathway, a positive regulator of cold tolerance in banana.PMC78909762018B0202020052015B07070101131872939201904020033, 2019030100102018LM21502018YFD1000302Dong TLi CGao JGao HDou TZhang SDeng GBi FHu CHe WSheng OYang QYi GfalseMaMAPK3-MaICE1-MaPOD P7 pathway, a positive regulator of cold tolerance in banana.<h4>Background</h4>Banana is a tropical fruit with a high economic impact worldwide. Cold stress greatly affects the development and production of banana.<h4>Results</h4>In the present study, we investigated the functions of MaMAPK3 and MaICE1 involved in cold tolerance of banana. The effect of RNAi of MaMAPK3 on Dajiao (Musa spp. 'Dajiao'; ABB Group) cold tolerance was evaluated. The leaves of the MaMAPK3 RNAi transgenic plants showed wilting and severe necrotic symptoms, while the wide-type (WT) plants remained normal after cold exposure. RNAi of MaMAPK3 significantly changed the expressions of the cold-responsive genes, and the oxidoreductase activity was significantly changed in WT plants, while no changes in transgenic plants were observed. MaICE1 interacted with MaMAPK3, and the expr2021-01-01T00:00:00Z2021 Feb2026-04-07T22:48:23.322Z2021-02-25T08:58:21ZS-EPMC78909763359683010.1186/s12870-021-02868-z