{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["He J"],"funding":["NIBIB NIH HHS","National Institutes of Health","NIH HHS"],"pagination":["e3295"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7900953"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["23(2)"],"pubmed_abstract":["<h4>Background</h4>Previously, we determined that four-branched histidine-lysine (HK) peptides were effective carriers of plasmids and small interfering RNA. In the present study, we compared several branched HK carriers and, in particular, two closely-related H3K4b and H3K(+H)4b peptides for their ability as carriers of mRNA. The H3K(+H)4b peptide differed from its parent analogue, H3K4b, by only a single histidine in each branch.<h4>Methods</h4>A series of four-branched HK peptides with varied sequences was synthesized on a solid-phase peptide synthesizer. The ability of these peptides to carry mRNA expressing luciferase to MDA-MB-231 cells was investigated. With gel retardation and heparin displacement assays, the stability of HK polyplexes was examined. We determined the intracellular "],"journal":["The journal of gene medicine"],"pubmed_title":["Location of a single histidine within peptide carriers increases mRNA delivery."],"pmcid":["PMC7900953"],"funding_grant_id":["R01‐EB028534","R01-EB028534","R01 EB028534"],"pubmed_authors":["Leng Q","He J","Xu S","Mixson AJ"],"additional_accession":[]},"is_claimable":false,"name":"Location of a single histidine within peptide carriers increases mRNA delivery.","description":"<h4>Background</h4>Previously, we determined that four-branched histidine-lysine (HK) peptides were effective carriers of plasmids and small interfering RNA. In the present study, we compared several branched HK carriers and, in particular, two closely-related H3K4b and H3K(+H)4b peptides for their ability as carriers of mRNA. The H3K(+H)4b peptide differed from its parent analogue, H3K4b, by only a single histidine in each branch.<h4>Methods</h4>A series of four-branched HK peptides with varied sequences was synthesized on a solid-phase peptide synthesizer. The ability of these peptides to carry mRNA expressing luciferase to MDA-MB-231 cells was investigated. With gel retardation and heparin displacement assays, the stability of HK polyplexes was examined. We determined the intracellular ","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Feb","modification":"2025-05-29T22:28:04.437Z","creation":"2025-05-29T22:28:04.437Z"},"accession":"S-EPMC7900953","cross_references":{"pubmed":["33171540"],"doi":["10.1002/jgm.3295"]}}