{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["462"],"submitter":["Shen X"],"funding":["National Institute of General Medical Sciences","National Science Foundation"],"pubmed_abstract":["Native capillary zone electrophoresis-mass spectrometry (CZE-MS) has attracted attentions for the characterization of monoclonal antibodies (mAbs) due to the potential of CZE for highly efficient separations of mAbs under native conditions as well as its compatibility with native electrospray ionization (ESI)-MS. However, the low sample loading capacity and limited separation resolution of native CZE for large proteins and protein complexes (e.g. mAbs) impede the widespread adoption of native CZE-MS. Here, we present a novel native capillary isoelectric focusing (cIEF)-assisted CZE-MS method for the characterization of mAbs with much larger sample loading capacity and significantly better separation resolution than native CZE-MS alone. The native cIEF-assisted CZE-MS employed separation ca"],"journal":["International journal of mass spectrometry"],"pagination":["116541"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7906288"],"repository":["biostudies-literature"],"pubmed_title":["Investigating native capillary zone electrophoresis-mass spectrometry on a high-end quadrupole-time-of-flight mass spectrometer for the characterization of monoclonal antibodies."],"pmcid":["PMC7906288"],"pubmed_authors":["Sun L","Yang Z","Wang Q","Liang Z","Xu T","Du W","Pham L","Shen X","Chen D"],"additional_accession":[]},"is_claimable":false,"name":"Investigating native capillary zone electrophoresis-mass spectrometry on a high-end quadrupole-time-of-flight mass spectrometer for the characterization of monoclonal antibodies.","description":"Native capillary zone electrophoresis-mass spectrometry (CZE-MS) has attracted attentions for the characterization of monoclonal antibodies (mAbs) due to the potential of CZE for highly efficient separations of mAbs under native conditions as well as its compatibility with native electrospray ionization (ESI)-MS. However, the low sample loading capacity and limited separation resolution of native CZE for large proteins and protein complexes (e.g. mAbs) impede the widespread adoption of native CZE-MS. Here, we present a novel native capillary isoelectric focusing (cIEF)-assisted CZE-MS method for the characterization of mAbs with much larger sample loading capacity and significantly better separation resolution than native CZE-MS alone. The native cIEF-assisted CZE-MS employed separation ca","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Apr","modification":"2025-04-29T11:30:15.235Z","creation":"2025-04-06T19:55:09.17Z"},"accession":"S-EPMC7906288","cross_references":{"pubmed":["33642939"],"doi":["10.1016/j.ijms.2021.116541"]}}