biostudies-literatureUpasani VHoward Hughes Medical InstituteWellcome Trust594813https://www.ebi.ac.uk/biostudies/studies/S-EPMC7907177biostudies-literatureUnknown11Dengue is an acute viral disease caused by dengue virus (DENV), which is transmitted by <i>Aedes</i> mosquitoes. Symptoms of DENV infection range from inapparent to severe and can be life-threatening. DENV replicates in primary immune cells such as dendritic cells and macrophages, which contribute to the dissemination of the virus. Susceptibility of other immune cells such as B cells to direct infection by DENV and their subsequent response to infection is not well defined. In a cohort of 60 Cambodian children, we showed that B cells are susceptible to DENV infection. Moreover, we show that B cells can support viral replication of laboratory adapted and patient-derived DENV strains. B cells were permissive to DENV infection albeit low titers of infectious virions were released in cell supeFrontiers in immunologyDirect Infection of B Cells by Dengue Virus Modulates B Cell Responses in a Cambodian Pediatric Cohort.PMC7907177208710/Z/17/ZAuerswald HDuong VUpasani VVo HTMHeng SRodenhuis-Zybert IALaurent DCantaert TDussart PfalseDirect Infection of B Cells by Dengue Virus Modulates B Cell Responses in a Cambodian Pediatric Cohort.Dengue is an acute viral disease caused by dengue virus (DENV), which is transmitted by <i>Aedes</i> mosquitoes. Symptoms of DENV infection range from inapparent to severe and can be life-threatening. DENV replicates in primary immune cells such as dendritic cells and macrophages, which contribute to the dissemination of the virus. Susceptibility of other immune cells such as B cells to direct infection by DENV and their subsequent response to infection is not well defined. In a cohort of 60 Cambodian children, we showed that B cells are susceptible to DENV infection. Moreover, we show that B cells can support viral replication of laboratory adapted and patient-derived DENV strains. B cells were permissive to DENV infection albeit low titers of infectious virions were released in cell supe2020-01-01T00:00:00Z20202026-04-07T21:32:56.931Z2021-03-02T08:09:58ZS-EPMC79071773364328310.3389/fimmu.2020.594813