biostudies-literatureAndersen BVillum Fonden71https://www.ebi.ac.uk/biostudies/studies/S-EPMC7909426biostudies-literatureUnknown7(2)In many complaint cases regarding bad indoor environments, there is no evidence of visible fungal growth. To determine if the problems are fungi-related, dust sampling is the method of choice among building surveyors. However, there is a need to differentiate between species belonging to a normal, dry indoor environment and species belonging to a damp building envelope. The purposes of this pilot study were to examine which fungal species are present in problem-free Danish homes and to evaluate different detection and identification methods. Analyses showed that the fungal diversity outside was different from the diversity inside and that the composition of fungal species growing indoors was different compared to those found as spores, both indoors and outdoors. Common for most homes were Journal of fungi (Basel, Switzerland)A Pilot Study on Baseline Fungi and Moisture Indicator Fungi in Danish Homes.PMC7909426-Dunn RRFrisvad JCThrane UAndersen BfalseA Pilot Study on Baseline Fungi and Moisture Indicator Fungi in Danish Homes.In many complaint cases regarding bad indoor environments, there is no evidence of visible fungal growth. To determine if the problems are fungi-related, dust sampling is the method of choice among building surveyors. However, there is a need to differentiate between species belonging to a normal, dry indoor environment and species belonging to a damp building envelope. The purposes of this pilot study were to examine which fungal species are present in problem-free Danish homes and to evaluate different detection and identification methods. Analyses showed that the fungal diversity outside was different from the diversity inside and that the composition of fungal species growing indoors was different compared to those found as spores, both indoors and outdoors. Common for most homes were 2021-01-01T00:00:00Z2021 Jan2025-05-29T22:28:23.154Z2025-05-29T22:28:23.154ZS-EPMC79094263349844610.3390/jof7020071