{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Deschler S"],"funding":["Deutsche Forschungsgemeinschaft","Bavarian State Ministry for Science and the Arts","Else Kröner-Fresenius-Stiftung"],"pagination":["241"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7913667"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13(2)"],"pubmed_abstract":["Coronavirus disease 2019 (COVID-19), caused by infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), comprises mild courses of disease as well as progression to severe disease, characterised by lung and other organ failure. The immune system is considered to play a crucial role for the pathogenesis of COVID-19, although especially the contribution of innate-like T cells remains poorly understood. Here, we analysed the phenotype and function of mucosal-associated invariant T (MAIT) cells, innate-like T cells with potent antimicrobial effector function, in patients with mild and severe COVID-19 by multicolour flow cytometry. Our data indicate that MAIT cells are highly activated in patients with COVID-19, irrespective of the course of disease, and express high levels of proinflammatory cytokines such as IL-17A and TNFα ex vivo. Of note, expression of the activation marker HLA-DR positively correlated with SAPS II score, a measure of disease severity. Upon MAIT cell-specific in vitro stimulation, MAIT cells however failed to upregulate expression of the cytokines IL-17A and TNFα, as well as cytolytic proteins, that is, granzyme B and perforin. Thus, our data point towards an altered cytokine expression profile alongside an impaired antibacterial and antiviral function of MAIT cells in COVID-19 and thereby contribute to the understanding of COVID-19 immunopathogenesis."],"journal":["Viruses"],"pubmed_title":["Mucosal-Associated Invariant T (MAIT) Cells Are Highly Activated and Functionally Impaired in COVID-19 Patients."],"pmcid":["PMC7913667"],"funding_grant_id":["Else Kröner Memorial Stipend","424774790","n.a."],"pubmed_authors":["Georgieva A","Protzer U","Horstmann J","Wiessner JR","Voit F","Bottcher K","Fricke L","Lahmer T","Erber J","Spinner CD","Schmid RM","Treiber M","Koyumdzhieva P","Quante M","Schneider J","Iakoubov R","Winter C","Knolle PA","Ruland J","Busch DH","Kager J","Deschler S"],"additional_accession":[]},"is_claimable":false,"name":"Mucosal-Associated Invariant T (MAIT) Cells Are Highly Activated and Functionally Impaired in COVID-19 Patients.","description":"Coronavirus disease 2019 (COVID-19), caused by infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), comprises mild courses of disease as well as progression to severe disease, characterised by lung and other organ failure. The immune system is considered to play a crucial role for the pathogenesis of COVID-19, although especially the contribution of innate-like T cells remains poorly understood. Here, we analysed the phenotype and function of mucosal-associated invariant T (MAIT) cells, innate-like T cells with potent antimicrobial effector function, in patients with mild and severe COVID-19 by multicolour flow cytometry. Our data indicate that MAIT cells are highly activated in patients with COVID-19, irrespective of the course of disease, and express high levels of proinflammatory cytokines such as IL-17A and TNFα ex vivo. Of note, expression of the activation marker HLA-DR positively correlated with SAPS II score, a measure of disease severity. Upon MAIT cell-specific in vitro stimulation, MAIT cells however failed to upregulate expression of the cytokines IL-17A and TNFα, as well as cytolytic proteins, that is, granzyme B and perforin. Thus, our data point towards an altered cytokine expression profile alongside an impaired antibacterial and antiviral function of MAIT cells in COVID-19 and thereby contribute to the understanding of COVID-19 immunopathogenesis.","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Feb","modification":"2024-11-13T03:46:06.573Z","creation":"2021-03-02T08:09:53Z"},"accession":"S-EPMC7913667","cross_references":{"pubmed":["33546489"],"doi":["10.3390/v13020241"]}}