{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Vitale DL"],"funding":["GLYCANC PROJECT - H2020"],"pagination":["246"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7914570"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["11(2)"],"pubmed_abstract":["UDP-glucose-dehydrogenase (UGDH) synthesizes UDP-glucuronic acid. It is involved in epirubicin detoxification and hyaluronan synthesis. This work aimed to evaluate the effect of UGDH knockdown on epirubicin response and hyaluronan metabolism in MDA-MB-231 breast cancer cells. Additionally, the aim was to determine UGDH as a possible prognosis marker in breast cancer. We studied UGDH expression in tumors and adjacent tissue from breast cancer patients. The prognostic value of UGDH was studied using a public Kaplan-Meier plotter. MDA-MB-231 cells were knocked-down for UGDH and treated with epirubicin. Epirubicin-accumulation and apoptosis were analyzed by flow cytometry. Hyaluronan-coated matrix and metabolism were determined. Autophagic-LC3-II was studied by Western blot and confocal micros"],"journal":["Biomolecules"],"pubmed_title":["Initial Identification of UDP-Glucose Dehydrogenase as a Prognostic Marker in Breast Cancer Patients, Which Facilitates Epirubicin Resistance and Regulates Hyaluronan Synthesis in MDA-MB-231 Cells."],"pmcid":["PMC7914570"],"funding_grant_id":["645756","0561/2019"],"pubmed_authors":["Vigetti D","Alaniz L","Parnigoni A","Caon I","Sevic I","Icardi A","Passi A","Spinelli FM","Vitale DL"],"additional_accession":[]},"is_claimable":false,"name":"Initial Identification of UDP-Glucose Dehydrogenase as a Prognostic Marker in Breast Cancer Patients, Which Facilitates Epirubicin Resistance and Regulates Hyaluronan Synthesis in MDA-MB-231 Cells.","description":"UDP-glucose-dehydrogenase (UGDH) synthesizes UDP-glucuronic acid. It is involved in epirubicin detoxification and hyaluronan synthesis. This work aimed to evaluate the effect of UGDH knockdown on epirubicin response and hyaluronan metabolism in MDA-MB-231 breast cancer cells. Additionally, the aim was to determine UGDH as a possible prognosis marker in breast cancer. We studied UGDH expression in tumors and adjacent tissue from breast cancer patients. The prognostic value of UGDH was studied using a public Kaplan-Meier plotter. MDA-MB-231 cells were knocked-down for UGDH and treated with epirubicin. Epirubicin-accumulation and apoptosis were analyzed by flow cytometry. Hyaluronan-coated matrix and metabolism were determined. Autophagic-LC3-II was studied by Western blot and confocal micros","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Feb","modification":"2025-04-26T05:58:33.048Z","creation":"2021-03-03T08:09:56Z"},"accession":"S-EPMC7914570","cross_references":{"pubmed":["33572239"],"doi":["10.3390/biom11020246"]}}