biostudies-literatureGong TScience and Technology Program of Guizhou ProvinceNational Natural Science Foundation of ChinaScientific Research Cooperation Project with America and Oceania Region, Ministry of Education437https://www.ebi.ac.uk/biostudies/studies/S-EPMC7916009biostudies-literatureUnknown11(2)Testicular expression of taste receptor type 1 subunit 3 (T1R3), a sweet/umami taste receptor, has been implicated in spermatogenesis and steroidogenesis in mice. We explored the role of testicular T1R3 in porcine postnatal development using the Congjiang Xiang pig, a rare Chinese miniature pig breed. Based on testicular weights, morphology, and testosterone levels, four key developmental stages were identified in the pig at postnatal days 15-180 (prepuberty: 30 day; early puberty: 60 day; late puberty: 90 day; sexual maturity: 120 day). During development, testicular T1R3 exhibited stage-dependent and cell-specific expression patterns. In particular, T1R3 levels increased significantly from prepuberty to puberty (<i>p</i> < 0.05), and expression remained high until sexual maturity (<i>p</Animals : an open access journal from MDPILongitudinal Expression of Testicular <i>TAS1R3</i> from Prepuberty to Sexual Maturity in Congjiang Xiang Pigs.PMC7916009JWSM[2016]2191QKHPTRC[2018]5436, QKHJC[2020]1Y135 and QKHJC[2020]1Z02831702117Wan SGong TYang YLi ZWang WMeng LChen XXu HMu QXu YfalseLongitudinal Expression of Testicular <i>TAS1R3</i> from Prepuberty to Sexual Maturity in Congjiang Xiang Pigs.Testicular expression of taste receptor type 1 subunit 3 (T1R3), a sweet/umami taste receptor, has been implicated in spermatogenesis and steroidogenesis in mice. We explored the role of testicular T1R3 in porcine postnatal development using the Congjiang Xiang pig, a rare Chinese miniature pig breed. Based on testicular weights, morphology, and testosterone levels, four key developmental stages were identified in the pig at postnatal days 15-180 (prepuberty: 30 day; early puberty: 60 day; late puberty: 90 day; sexual maturity: 120 day). During development, testicular T1R3 exhibited stage-dependent and cell-specific expression patterns. In particular, T1R3 levels increased significantly from prepuberty to puberty (<i>p</i> < 0.05), and expression remained high until sexual maturity (<i>p</2021-01-01T00:00:00Z2021 Feb2026-04-18T01:20:22.99Z2021-03-03T08:08:17ZS-EPMC79160093356755510.3390/ani11020437