{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Sedlar A"],"funding":["Ministerstvo Zdravotnictví Ceské Republiky","Grantová Agentura České Republiky","Ministerstvo Školství, Mládeže a Tělovýchovy"],"pagination":["1843"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7917819"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["22(4)"],"pubmed_abstract":["Vascular endothelial growth factor-A165 (VEGF-A165) and fibroblast growth factor-2 (FGF-2) are currently used for the functionalization of biomaterials designed for tissue engineering. We have developed a new simple method for heterologous expression and purification of VEGF-A165 and FGF-2 in the yeast expression system of Pichia pastoris. The biological activity of the growth factors was assessed in cultures of human and porcine adipose tissue-derived stem cells (ADSCs) and human umbilical vein endothelial cells (HUVECs). When added into the culture medium, VEGF-A165 stimulated proliferation only in HUVECs, while FGF-2 stimulated the proliferation of both cell types. A similar effect was achieved when the growth factors were pre-adsorbed to polystyrene wells. The effect of our recombinant"],"journal":["International journal of molecular sciences"],"pubmed_title":["Growth Factors VEGF-A165 and FGF-2 as Multifunctional Biomolecules Governing Cell Adhesion and Proliferation."],"pmcid":["PMC7917819"],"funding_grant_id":["BIOCEV-FAR Project within LQ1604 National Sustainability Program II","LTC18038","18-01163S","NV18-02-00422","CZ.1.05/1.1.00/02.0109"],"pubmed_authors":["Travnickova M","Matejka R","Sedlar A","Prazak S","Kren V","Meszaros Z","Bojarova P","Slamova K","Bacakova L"],"additional_accession":[]},"is_claimable":false,"name":"Growth Factors VEGF-A165 and FGF-2 as Multifunctional Biomolecules Governing Cell Adhesion and Proliferation.","description":"Vascular endothelial growth factor-A165 (VEGF-A165) and fibroblast growth factor-2 (FGF-2) are currently used for the functionalization of biomaterials designed for tissue engineering. We have developed a new simple method for heterologous expression and purification of VEGF-A165 and FGF-2 in the yeast expression system of Pichia pastoris. The biological activity of the growth factors was assessed in cultures of human and porcine adipose tissue-derived stem cells (ADSCs) and human umbilical vein endothelial cells (HUVECs). When added into the culture medium, VEGF-A165 stimulated proliferation only in HUVECs, while FGF-2 stimulated the proliferation of both cell types. A similar effect was achieved when the growth factors were pre-adsorbed to polystyrene wells. The effect of our recombinant","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Feb","modification":"2025-04-26T05:56:46.937Z","creation":"2025-04-06T11:37:37.063Z"},"accession":"S-EPMC7917819","cross_references":{"pubmed":["33673317"],"doi":["10.3390/ijms22041843"]}}