biostudies-literatureSedlar AMinisterstvo Zdravotnictví Ceské RepublikyGrantová Agentura České RepublikyMinisterstvo Školství, Mládeže a Tělovýchovy1843https://www.ebi.ac.uk/biostudies/studies/S-EPMC7917819biostudies-literatureUnknown22(4)Vascular endothelial growth factor-A165 (VEGF-A165) and fibroblast growth factor-2 (FGF-2) are currently used for the functionalization of biomaterials designed for tissue engineering. We have developed a new simple method for heterologous expression and purification of VEGF-A165 and FGF-2 in the yeast expression system of Pichia pastoris. The biological activity of the growth factors was assessed in cultures of human and porcine adipose tissue-derived stem cells (ADSCs) and human umbilical vein endothelial cells (HUVECs). When added into the culture medium, VEGF-A165 stimulated proliferation only in HUVECs, while FGF-2 stimulated the proliferation of both cell types. A similar effect was achieved when the growth factors were pre-adsorbed to polystyrene wells. The effect of our recombinantInternational journal of molecular sciencesGrowth Factors VEGF-A165 and FGF-2 as Multifunctional Biomolecules Governing Cell Adhesion and Proliferation.PMC7917819BIOCEV-FAR Project within LQ1604 National Sustainability Program IILTC1803818-01163SNV18-02-00422CZ.1.05/1.1.00/02.0109Travnickova MMatejka RSedlar APrazak SKren VMeszaros ZBojarova PSlamova KBacakova LfalseGrowth Factors VEGF-A165 and FGF-2 as Multifunctional Biomolecules Governing Cell Adhesion and Proliferation.Vascular endothelial growth factor-A165 (VEGF-A165) and fibroblast growth factor-2 (FGF-2) are currently used for the functionalization of biomaterials designed for tissue engineering. We have developed a new simple method for heterologous expression and purification of VEGF-A165 and FGF-2 in the yeast expression system of Pichia pastoris. The biological activity of the growth factors was assessed in cultures of human and porcine adipose tissue-derived stem cells (ADSCs) and human umbilical vein endothelial cells (HUVECs). When added into the culture medium, VEGF-A165 stimulated proliferation only in HUVECs, while FGF-2 stimulated the proliferation of both cell types. A similar effect was achieved when the growth factors were pre-adsorbed to polystyrene wells. The effect of our recombinant2021-01-01T00:00:00Z2021 Feb2025-04-26T05:56:46.937Z2025-04-06T11:37:37.063ZS-EPMC79178193367331710.3390/ijms22041843