{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kern U"],"funding":["Deutsche Forschungsgemeinschaft"],"pagination":["404"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7920061"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["10(2)"],"pubmed_abstract":["DJ-1 is an abundant and ubiquitous component of cellular proteomes. DJ-1 supposedly exerts a wide variety of molecular functions, ranging from enzymatic activities as a deglycase, protease, and esterase to chaperone functions. However, a consensus perspective on its molecular function in the cellular context has not yet been reached. Structurally, the C-terminal helix 8 of DJ-1 has been proposed to constitute a propeptide whose proteolytic removal transforms a DJ-1 zymogen to an active hydrolase with potential proteolytic activity. To better understand the cell-contextual functionality of DJ-1 and the role of helix 8, we employed post-mitotically differentiated, neuron-like SH-SY5Y neuroblastoma cells with stable over-expression of full length DJ-1 or DJ-1 lacking helix 8 (ΔH8), either wit"],"journal":["Cells"],"pubmed_title":["Impact of DJ-1 and Helix 8 on the Proteome and Degradome of Neuron-Like Cells."],"pmcid":["PMC7920061"],"funding_grant_id":["SCHI 871/11-1; PA 2807/3-1; SCHI 871/9-1; SCHI 871/8-1;GR 1748/6-1;","SFB850"],"pubmed_authors":["Schmidt N","Gensch N","Frohlich K","Biniossek ML","Kern U","Baerenfaller K","Schilling O","Bedacht J"],"additional_accession":[]},"is_claimable":false,"name":"Impact of DJ-1 and Helix 8 on the Proteome and Degradome of Neuron-Like Cells.","description":"DJ-1 is an abundant and ubiquitous component of cellular proteomes. DJ-1 supposedly exerts a wide variety of molecular functions, ranging from enzymatic activities as a deglycase, protease, and esterase to chaperone functions. However, a consensus perspective on its molecular function in the cellular context has not yet been reached. Structurally, the C-terminal helix 8 of DJ-1 has been proposed to constitute a propeptide whose proteolytic removal transforms a DJ-1 zymogen to an active hydrolase with potential proteolytic activity. To better understand the cell-contextual functionality of DJ-1 and the role of helix 8, we employed post-mitotically differentiated, neuron-like SH-SY5Y neuroblastoma cells with stable over-expression of full length DJ-1 or DJ-1 lacking helix 8 (ΔH8), either wit","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Feb","modification":"2026-04-07T22:43:59.176Z","creation":"2025-05-29T20:31:52.74Z"},"accession":"S-EPMC7920061","cross_references":{"pubmed":["33669258"],"doi":["10.3390/cells10020404"]}}