biostudies-literatureKern UDeutsche Forschungsgemeinschaft404https://www.ebi.ac.uk/biostudies/studies/S-EPMC7920061biostudies-literatureUnknown10(2)DJ-1 is an abundant and ubiquitous component of cellular proteomes. DJ-1 supposedly exerts a wide variety of molecular functions, ranging from enzymatic activities as a deglycase, protease, and esterase to chaperone functions. However, a consensus perspective on its molecular function in the cellular context has not yet been reached. Structurally, the C-terminal helix 8 of DJ-1 has been proposed to constitute a propeptide whose proteolytic removal transforms a DJ-1 zymogen to an active hydrolase with potential proteolytic activity. To better understand the cell-contextual functionality of DJ-1 and the role of helix 8, we employed post-mitotically differentiated, neuron-like SH-SY5Y neuroblastoma cells with stable over-expression of full length DJ-1 or DJ-1 lacking helix 8 (ΔH8), either witCellsImpact of DJ-1 and Helix 8 on the Proteome and Degradome of Neuron-Like Cells.PMC7920061SCHI 871/11-1; PA 2807/3-1; SCHI 871/9-1; SCHI 871/8-1;GR 1748/6-1;SFB850Schmidt NGensch NFrohlich KBiniossek MLKern UBaerenfaller KSchilling OBedacht JfalseImpact of DJ-1 and Helix 8 on the Proteome and Degradome of Neuron-Like Cells.DJ-1 is an abundant and ubiquitous component of cellular proteomes. DJ-1 supposedly exerts a wide variety of molecular functions, ranging from enzymatic activities as a deglycase, protease, and esterase to chaperone functions. However, a consensus perspective on its molecular function in the cellular context has not yet been reached. Structurally, the C-terminal helix 8 of DJ-1 has been proposed to constitute a propeptide whose proteolytic removal transforms a DJ-1 zymogen to an active hydrolase with potential proteolytic activity. To better understand the cell-contextual functionality of DJ-1 and the role of helix 8, we employed post-mitotically differentiated, neuron-like SH-SY5Y neuroblastoma cells with stable over-expression of full length DJ-1 or DJ-1 lacking helix 8 (ΔH8), either wit2021-01-01T00:00:00Z2021 Feb2026-04-07T22:43:59.176Z2025-05-29T20:31:52.74ZS-EPMC79200613366925810.3390/cells10020404